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Key Documents

安全性情報

C0581

Sigma-Aldrich

モノクロナール抗カルシニューリン (β-サブユニット) マウス宿主抗体

clone CN-B1, ascites fluid

別名:

Anti-CALNB1, Anti-CNB, Anti-CNB1

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About This Item

MDL番号:
UNSPSCコード:
12352203
NACRES:
NA.44

由来生物

mouse

結合体

unconjugated

抗体製品の状態

ascites fluid

抗体製品タイプ

primary antibodies

クローン

CN-B1, monoclonal

含みます

15 mM sodium azide

化学種の反応性

bovine, rat, human, mouse

テクニック

immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable using neurons in human myenteric and prostatic ganglia
indirect ELISA: suitable
western blot: 1:3,000 using rat brain extract

アイソタイプ

IgG2b

UniProtアクセッション番号

輸送温度

dry ice

保管温度

−20°C

ターゲットの翻訳後修飾

unmodified

遺伝子情報

詳細

Calcineurin (CaN, heterodimeric enzyme) is a Ca(2+)/calmodulin (CaM)-stimulated protein phosphatase 3 (PPP3) consisting of a catalytic A subunit (CnA) and a Ca(2+)-binding regulatory B subunit (CnB). The calcium-binding regulatory subunit (calcineurin B) is coded by the PPP3R1 gene located on human chromosome 2. Protein phosphatase 3, regulatory subunit B, α (PPP3R1) is also known as CALN, CCN1, CNA1, CALNA, PPP2B and CALNA1. The regulatory subunit (calcineurin B) is widely expressed in brain while the catalytic subunit (calcineurin A) is abundantly expressed in Hassall′s corpuscles, localized in the thymic medulla and represents the terminal stages of thymic medullary epithelium.
Monoclonal Anti-Calcineurin (β-subunit) (mouse IgM isotype) is derived from the CN-B1 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with purified bovine brain calcineurin preparation. Two different calcineurin B has been reported in rat testis.

特異性

抗体は、イムノブロッティングにおいてカルシニュ-リンのβ-サブユニット(18 kDa, 別名カルシニュ-リンB)に位置するエピト-プを認識しますが、α-サブユニットとの交差反応性はありません。

免疫原

ウシ脳カルシニュ-リン。

アプリケーション

Monoclonal Anti-Calcineurin (b-Subunit) antibody produced in mouse has been used in immunohistochemistry, indirect ELISA and western blotting.

生物化学的/生理学的作用

Calcineurin calcium -binding subunit, calcineurin B, is immunologically conserved from yeast to mammals. immunoreactivity is detected at significant concentrations only in normal and neoplastic neurons regions of the cytoplasm dendrites, axons and axon terminals. A close correlation has been observed between inhibition of calcineurin by the complexes of cyclosporin A/cyclophilin and tacrolimus(FK506) and FK506 binding proteins (FKBPs) and inhibition of apoptosis. Calcineurin is an excellent marker enzyme for the detection of neuronal activity and synaptic plasticity after brain damage, such as an ischemic insult.
Calcineurin is a major soluble calmodulin-binding protein in the brain. It stimulates the expression of the surface molecules CD83, CD80, CD86, CD40 and HLA-DR by promoting secretion of inflammatory cytokines IL-6, TNF-α and IL-1β by human PBMC-derived dendritic cells. It plays an important role in signal transduction, activation of T cell. Calcineurin is an excellent marker enzyme for the detection of neuronal activity and synaptic plasticity after brain damage.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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保管分類コード

10 - Combustible liquids

WGK

WGK 3

引火点(°F)

Not applicable

引火点(℃)

Not applicable


適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

Jan Code

C0581-.2ML:
C0581-VAR:
C0581-BULK:
C0581-.5ML:


試験成績書(COA)

製品のロット番号・バッチ番号を入力して、試験成績書(COA) を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。

以前この製品を購入いただいたことがある場合

文書ライブラリで、最近購入した製品の文書を検索できます。

文書ライブラリにアクセスする

The unique C terminus of the calcineurin isoform CNAbeta1 confers non-canonical regulation of enzyme activity by Ca2+ and calmodulin
Bond R, et al.
The Journal of biological chemistry, 292(40), 16709-16721 (2017)
Rachel Bond et al.
The Journal of biological chemistry, 292(40), 16709-16721 (2017-08-27)
Calcineurin, the conserved Ca2+/calmodulin-regulated phosphatase and target of immunosuppressants, plays important roles in the circulatory, nervous, and immune systems. Calcineurin activity strictly depends on Ca2+ and Ca2+-bound calmodulin (Ca2+/CaM) to relieve autoinhibition of the catalytic subunit (CNA) by its C
Substitution in position 3 of cyclosporin A abolishes the cyclophilin-mediated gain-of-function mechanism but not immunosuppression
Baumgrass R, et al.
The Journal of Biological Chemistry, 279(4), 2470-2479 (2004)
Neural functions of calcineurin in synaptic plasticity and memory
Baumgartel K and Mansuy IM
Learning & Memory, 19(9), 375-384 (2012)
Protein phosphatase 2B (PP2B, calcineurin) in Paramecium: partial characterization reveals that two members of the unusually large catalytic subunit family have distinct roles in calcium-dependent processes
Fraga D, et al.
Eukaryotic Cell, 9(7), 1049-1063 (2010)

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