A1106

Anti-ATM antibody,Mouse monoclonal

clone MAT3-4G10/8, purified from hybridoma cell culture

• 別名: Anti-Ataxia-Telangiectasia Mutated
• MDL番号: MFCD01321740
• UNSPSCコード: 12352203
• NACRES: NA.45

特性

• 由来生物: mouse
• 品質水準: 200
• リコンビナント: expressed in mouse cell line
• 結合体: unconjugated
• 抗体製品の状態: purified from hybridoma cell culture
• 抗体製品タイプ: primary antibodies
• クローン: MAT3-4G10/8, monoclonal
• 形状: PBS solution
• 分子量: antigen ~300 kDa
• 化学種の反応性: human, mouse
• 包装: antibody small pack of 25 μL
• 濃度: ~2 mg/mL
• テクニック: immunoprecipitation (IP): suitable; indirect ELISA: suitable; western blot: 0.1-0.2 μg/mL using HEK-293T total cel extract
• アイソタイプ: IgG1
• UniProtアクセッション番号: Q13315
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... ATM(472) ; mouse ... Atm(11920)

詳細

詳細

Monoclonal Anti-ATM (mouse IgG1 isotype) is derived from the hybridoma MAT3-4G10/8 produced by the fusion of mouse myeloma cells (NSO) and splenocytes from BALB/c mice immunized with a peptide.

特異性

Monoclonal Anti-ATM antibody reacts specifically with mouse and human ATM.

免疫原

マウスATMのN末端システインを含む膜貫通位置のアミノ酸1967-1988に相当するペプチドのKLH結合体。

アプリケーション

Monoclonal Anti-ATM antibody can be used in immunoblotting , ELISA and immunoprecipitation.

生物化学的／生理学的作用

Anti-Ataxia-Telangiectasia Mutated (ATM) is responsible for the activation and stabilization of p53 in response to double-strand break (DSB). ATM phosphorylates p53 directly on Ser15 and concomitantly activates other kinases that phosphorylate the same molecule on additional sites. Furthermore, human homolog of double minute 2 (Hdm2) is phosphorylated by ATM on Ser395 and this phosphorylation inhibits Hdm2-mediated degradation of p53.

物理的形状

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable