A0420

抗ヒトIgM (μ-鎖特異的)−ペルオキシダーゼ ヤギ宿主抗体

affinity isolated antibody

• 別名: Goat Anti-Human IgM (μ-chain specific)−HRP
• MDL番号: MFCD00162464
• UNSPSCコード: 12352203
• NACRES: NA.46

特性

• 由来生物: goat
• 品質水準: 300
• 結合体: peroxidase conjugate
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: secondary antibodies
• クローン: polyclonal
• 化学種の反応性: human
• テクニック: direct ELISA: 1:50,000; dot blot: 1:100,000 (chemiluminescent); immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified

詳細

詳細

B cells predominantly secrete IgM antibodies as pentamers, but may also secrete them as hexamers which are capable of activating the complement cascade more efficiently.
Goat anti-human IgM (mu-chain specific)-peroxidase antibody is specific for human IgM and does not bind to other human Igs. Furthermore, the antibody does not react with mouse and rat IgG. Hence, the antibody conjugate yields reduced background with mouse or rat samples.

免疫原

human IgM

アプリケーション

Anti- Human IgM was used in an ELISA assay to determine the concentration of IgM secreted by CH12 B lymphoma cells stimulated by LPS.

Goat anti-human IgM (mu-chain specific)-peroxidase antibody can be used:

• for dot blot (1:100,000) and immunohistochemistry (1:200 using formalin-fixed, paraffin-embedded sections) applications.
• in immunoblotting
• sandwich immunoassay

生物化学的／生理学的作用

IgM plays a key role in engulfing of the apoptotic cells. A reduction in serum IgM levels leads to increased autoimmune response and higher risk for infections. IgM displays polyreactive and autoreactive functionality. It plays a key role in tissue homeostasis by mediating clearance of tissue based molecules.

物理的形状

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 0.05% MIT

調製ノート

Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978)によって報告された2段階グルタルアルデヒド法により作製しています。

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• ピクトグラム: GHS07
• シグナルワード: Warning
• 危険有害性情報: H317
• 注意書き: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• 危険有害性の分類: Skin Sens. 1
• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable
• 個人用保護具 (PPE): Eyeshields, Gloves