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Merck

5006

Sigma-Aldrich

PureCol

Collagen powder from Bovine hide, 97% Type I with the remainder of Type III collagen, suitable for cell culture

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About This Item

EC Number:
UNSPSCコード:
12352202
NACRES:
NA.75

品質水準

無菌性

sterile-filtered

アッセイ

99.9%

形状

lyophilized

包装

pkg of 15 mg

テクニック

cell culture | mammalian: suitable

結合特異性

Peptide Source: Elastin

Peptide Source: Fibrinogen

輸送温度

wet ice

保管温度

−20°C

関連するカテゴリー

詳細

PureCol collagen is approximately 97% Type I with the remainder being comprised of Type III collagen. It is soluble atelo-collagen. It contains high monomer content as measured by gel permeation chromatography.

It is ideal for coating of surfaces and providing preparations of thin layers for culturing cells. PureCol, lyophilized form is not recommended for the formation of a solid gel. PureCol collagen is provided in user-friendly packaging for use and storage. This product is supplied as a sterile lyophilized powder.

アプリケーション

PureCol has been used:
  • in collagen gel preparation for the 3D sprouting assay with human umbilical vein endothelial cells (HUVEC) spheroids 
  • to coat Costar® Transwell® Inserts plates before cell migration assays with bladder smooth muscle cells (BMSC)
  • to coat culture plates for collagen degradation assay with breast cancer cell lines

調製ノート

This product is supplied as a lyophilized powder with 15 mg of bovine collagen. When reconstituted with 5 ml of sterile 0.01 N HCl, a concentration of approximately 3 mg/ml is achieved.

法的情報

Costar is a registered trademark of Corning, Inc.
PureCol is a trademark of Advanced BioMatrix, Inc.
Transwell is a registered trademark of Corning, Inc.

保管分類コード

11 - Combustible Solids

WGK

WGK 1

引火点(°F)

Not applicable

引火点(℃)

Not applicable


試験成績書(COA)

製品のロット番号・バッチ番号を入力して、試験成績書(COA) を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。

以前この製品を購入いただいたことがある場合

文書ライブラリで、最近購入した製品の文書を検索できます。

文書ライブラリにアクセスする

Rebecca Long Heise et al.
Tissue engineering. Part A, 15(12), 3951-3960 (2009-07-03)
Successful approaches to tissue engineering smooth muscle tissues utilize biodegradable scaffolds seeded with autologous cells. One common problem in using biological scaffolds specifically is the difficulty of inducing cellular penetration and controlling de novo extracellular matrix deposition/remodeling in vitro. Our

資料

3D cell culture overview. Learn about 2D vs 3D cell culture, advantages of 3D cell culture, and techniques available to develop 3D cell models

3D cell culture overview: 3D cell cultures are increasingly being used for basic research and drug discovery. Learn about 2D vs 3D cell culture, advantage of 3D cell culture, and what about the techniques available to develop your 3D cell model.

Extracellular matrix proteins such as laminin, collagen, and fibronectin can be used as cell attachment substrates in cell culture.

Extracellular matrix proteins such as laminin, collagen, and fibronectin can be used as cell attachment substrates in cell culture.

ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.

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