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詳細
Merck USA index - 14, 8468
アッセイ
≥95% (cellulose acetate electrophoresis)
フォーム
powder
メーカー／製品名
Calbiochem®
保管条件
OK to freeze
不純物
≤0.02% fatty acids
≤3% ash
≤5% moisture
色
white to light yellow
light yellow to light brown
輸送温度
ambient
保管温度
2-8°C
詳細
Albumin, Bovine Serum, (BSA) Fraction V, Fatty Acid-Free is useful for immunodiagnostic assays. It is designed for use in serological testing, radioimmunoassay, and hormone response studies. It is also applicable in binding and transport studies. BSA is prepared by cold ethanol extraction (Cohn method) with a pH: 6.8-7.4.
Bovine serum albumin (BSA) is a non-glycosylated globular, α-helical protein belonging to the serum albumin family. It consists of three domains with two sub-domains each and has 17-disulfide bonds.
アプリケーション
Albumin, Bovine Serum, Fraction V, Fatty Acid-Free has been used:
- as a component of: blocking buffer immunoelectron microscopy (IEM) for various cells
- mitochondrial isolation buffer (MIB) and mitochondria assay solution (MAS) to trap free fatty acids released during adipose tissue homogenization
- blocking buffer for wholemount staining of the esophageal epithelium (EE) in immunofluorescence
生物化学的／生理学的作用
Bovine Serum Albumin (BSA), the most vital component of cell culture media, aids in embryonic stem cells (hESC) differentiation. It aids the transportation of drugs, hormones, and fatty acids. BSA is also used as a blocking agent in enzyme-linked immunosorbent assay (ELISA).
警告
Toxicity: Standard Handling (A)
調製ノート
May take up to 24 h to fully dissolve.
再構成
Following reconstitution, store in the refrigerator (4°C). 0.1% NaN₃ may be added as a preservative. Stock solutions are stable for up to 3 months at 4°C.
法的情報
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
保管分類コード
11 - Combustible Solids
WGK
WGK 3
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
126575-10GM:
126575-100GM:
126575-10KG:
126575-0GM:
126575-GM:
126575-1KG:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
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T Akao et al.
Journal of biochemistry, 99(5), 1425-1431 (1986-05-01)
Ruminococcus sp. PO1-3 obtained from human intestinal flora is able to reduce dehydrocholate as well as 3-ketoglycyrrhetinate. From this bacterium dehydrocholate- and 3-ketoglycyrrhetinate-reducing activities were purified one thousand-fold together with 3-ketocholanate-reducing and 3-beta-hydroxyglycyrrhetinate (glycyrrhetic acid) oxidizing activities by means of
M Prats
European journal of biochemistry, 75(2), 619-625 (1977-05-16)
The L-(+)-Lactate:cytochrome c oxidoreductase or cytochrome b2 from the yeasts Saccharomyces cerevisiae and Hansenula anomala were partially hydrolysed in various concentrations of trypsin. Conditions were found which allowed the isolation from the Hansenula enzyme of a 140 000 +/- 10
Alexandros Tzatsos et al.
Molecular and cellular biology, 26(1), 63-76 (2005-12-16)
Nutritional excess and/or obesity represent well-known predisposition factors for the development of non-insulin-dependent diabetes mellitus (NIDDM). However, molecular links between obesity and NIDDM are only beginning to emerge. Here, we demonstrate that nutrients suppress phosphatidylinositol 3 (PI3)-kinase/Akt signaling via Raptor-dependent
Saeko Akiyama et al.
Stem cell research & therapy, 13(1), 104-104 (2022-03-14)
The liver plays an important role in various metabolic processes, including protein synthesis, lipid and drug metabolisms and detoxifications. Primary culture of hepatocytes is used for the understanding of liver physiology as well as for the drug development. Hepatocytes are
Erica L Scheller et al.
Nature communications, 6, 7808-7808 (2015-08-08)
Marrow adipose tissue (MAT) accumulates in diverse clinical conditions but remains poorly understood. Here we show region-specific variation in MAT adipocyte development, regulation, size, lipid composition, gene expression and genetic determinants. Early MAT formation in mice is conserved, whereas later
資料
16HBE14o- human bronchial epithelial cells used to model respiratory epithelium for the research of cystic fibrosis, viral pulmonary pathology (SARS-CoV), asthma, COPD, effects of smoking and air pollution. See over 5k publications.
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