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Key Documents

安全性情報

5162

Sigma-Aldrich

Bovine Collagen Type I

from bovine flexor tendon, powder, suitable for cell culture, used for 3D gel formation

別名:

Collagen Protein, Collagen Solution, Collagen Type I

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About This Item

EC Number:
UNSPSCコード:
12352202
NACRES:
NA.75

product name

Collagen, from bovine flexor tendon

由来生物

bovine flexor tendon

無菌性

non-sterile

アッセイ

>96%

形状

lyophilized powder (fibrous)

包装

pkg of 1 g

テクニック

cell culture | mammalian: suitable

UniProtアクセッション番号

結合特異性

Peptide Source: Elastin

Peptide Source: Fibronectin

Peptide Source: Laminin

輸送温度

wet ice

保管温度

room temp

遺伝子情報

bovine ... COL2A1(407142)

詳細

This product is supplied as a lyophilized fibrous powder in a 1 gram package size. Bioburden and endotoxin levels are tested – this product is not considered sterile.

This collagen product is naturally cross-linked yielding a robust material for applications which require structure and strength. This product can be readily prepared into such forms as tissue scaffolds, foams, sponges, suspensions, coatings, putties, films and sheets but does not form hydrogels. Using typical cross-linking methods, this material can be tuned for optimal in vivo resorption. This collagen product is ideal for tissue engineering applications and uses with inorganic and biomaterials.

The product is provided in user-friendly packaging for use and storage. Avoid extended exposure to ambient environment since this material is hygroscopic.

調製ノート

Type I bovine collagen, insoluble lyophilized fibrous powder, is extracted from bovine flexor tendon with the raw material sourced from closed/controlled herds of animals. This product is very difficult to dissolve, and after following the below protocol you will end up with a viscous solution.
Note: The following procedure is based on solubilization of 1 gram of collagen in 100 ml to initially prepare a 10 mg/ml collagen suspension. Smaller quantities and volumes may be used but the same ratios of collagen and solutions should be used.
1. Weigh out 1 gram of collagen fibrous powder.
2. Reconstitute 1 gram of collagen with 50 ml of cold purified water (50% of the final volume).
3. Stir the collagen with the water continuously mixing for a minimum of 15 minutes until the collagen is fully wetted and the suspension appears to be a semi-solution.
4. Add 50 ml of cold 0.02 M HCl to the collagen mixture and stir for a minimum of 10 minutes. This will yield a collagen concentration of 10 mg/ml with the suspension continuing to appear as a semi-solution.
5. Measure the pH - the mixture should have a pH of 2 to 3.
6. Using Waring stick blender or equivalent, homogenize the collagen mixture for a minimum of 15 minute at a high speed ensuring that the collagen is fully homogenized. Ensure that the temperature of mixture does exceed 24°C. Upon completion, there should be no visual solids in the viscous suspension. Air bubbles with be prevalent.
7. To remove air bubbles, stir the solution on a stir plate and pull a vacuum on the suspension. This will remove the air bubbles.
8. At this point if a collagen concentration of less than 10 mg/ml is desired, the collagen can be diluted with 0.01 M HCl.

その他情報

Simultaneously vacuum filter 12 samples for rapid analysis of retentate or filtrate. Numbered positions on the filter plate assure accurate sample tracking. Sealing stoppers maintain vacuum on unused sample cups. Accessory extension barrels increase sample volumes from 15 to 50 mL.



Applications

General Filtration of 15 to 50 mL Samples, Preparation for Scintillation Counting



XX2702550 1225 Sampling Manifold



Replacement Parts

YY2214257 Hand Knob

5162 Top Plate

XX2702509 Filter O-ring, silicone

5163 Support Plate

XX2702510 Support Screen, 25 mm, polypropylene

XX11000PP Ball Valve, glass-filled polypropylene

XX2504755 Tubing, 1/4 in. x 23 in., latex



Accessories

XX2702552 Chamber O-rings and Plug Set

XX2702555 Sample Cup Extension Barrel

XX6200006P Filter Forceps, blunt end, stainless steel

XX7100004 Tubing 3/16 in. I.D. x 140 cm, silicone

保管分類コード

11 - Combustible Solids

WGK

WGK 1

引火点(°F)

Not applicable

引火点(℃)

Not applicable


適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

Jan Code

5162-1G:


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