47989

Fura 2-AM

BioReagent, suitable for fluorescence, ≥95.0% (HPLC)

• 別名: Fura-2ペンタキス(アセトキシメチル) エステル
• 実験式（ヒル表記法）: C₄₄H₄₇N₃O₂₄
• CAS番号: 108964-32-5
• 分子量: 1001.85
• Beilstein: 8183748
• MDL番号: MFCD00036976
• UNSPSCコード: 12352200
• PubChem Substance ID: 57651141
• NACRES: NA.32

特性

• 製品種目: BioReagent
• 品質水準: 100
• アッセイ: ≥95.0% (HPLC)
• 形状: solid
• 溶解性: DMSO: soluble
• 蛍光検出: λ_ex 355 nm; λ_em 495 nm in 0.1 M Tris pH 8.0, esterase; 10 mM Ca²⁺
• 適合性: suitable for fluorescence
• 保管温度: −20°C
• SMILES記法: CC(=O)OCOC(=O)CN(CC(=O)OCOC(C)=O)c1ccc(C)cc1OCCOc2cc3cc(oc3cc2N(CC(=O)OCOC(C)=O)CC(=O)OCOC(C)=O)-c4ncc(o4)C(=O)OCOC(C)=O
• InChI: 1S/C44H47N3O24/c1-25-7-8-32(46(16-39(53)65-20-60-26(2)48)17-40(54)66-21-61-27(3)49)35(11-25)58-9-10-59-36-12-31-13-37(43-45-15-38(71-43)44(57)69-24-64-30(6)52)70-34(31)14-33(36)47(18-41(55)67-22-62-28(4)50)19-42(56)68-23-63-29(5)51/h7-8,11-15H,9-10,16-24H2,1-6H3
• InChI Key: VPSRLGDRGCKUTK-UHFFFAOYSA-N

詳細

詳細

Fura 2-AM (Fura 2), also known as acetoxy-methyl-ester, is a calcium-specific ratio-metric fluorescent dye. In response to calcium ions, ratio metric dyes like Fura 2 changes their respective excitation or emission spectra. The intracellular calcium concentration is determined by the fluorescence emission ratio or excitation at distinct wavelengths. Fura 2 has an emission peak at 505nm, and the excitation peak ranges between 340-380nm and is the preferred calcium indicator.

アプリケーション

Fura 2-AM is a popular probe used to determine dynamic changes in cytosolic free calcium in intact living cells.

特徴および利点

The major benefits of using the ester form of Fura 2 as Fura 2-AM are:

• It is cell-permeable.
• It can be loaded into cells easily.

Other advantages of using Fura 2-AM over single wavelength dyes is that the ratio signal is independent of :

• The dye concentration.
• Illumination intensity, and
• Optical path length.

その他情報

Fura-2の膜透過性誘導体です。細胞内にキレ-ト剤を取り込む際に使用します。細胞中で酵素的に加水分解されて生じたFura-2が細胞内に残留します。

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable