344905

FURA 2/AM

Cell-permeable ester form of FURA 2.

• 別名: FURA 2/AM, 1-[2-(5-Carboxyoxazol-2-yl)-6-aminobenzofuran-5-oxy]-2-(2ʹ-amino-5ʹ-methylphenoxy)-ethane-N,N,NʹNʹ-tetraacetic Acid Pentaacetoxymethyl Ester
• 実験式（ヒル表記法）: C₄₄H₄₇N₃O₂₄
• CAS番号: 108964-32-5
• 分子量: 1001.85
• MDL番号: MFCD00036976
• UNSPSCコード: 12171500
• NACRES: NA.47

特性

• 品質水準: 100
• アッセイ: ≥95% (HPLC)
• 形状: solid
• メーカー／製品名: Calbiochem^®
• 保管条件: OK to freeze; protect from light
• 色: yellow
• 蛍光検出: λ_ex 335 nm (high); λ_ex 362 nm (low); λ_em 505 nm (high); λ_em 512 nm (low)
• 輸送温度: ambient
• 保管温度: −20°C
• InChI: 1S/C44H47N3O24/c1-25-7-8-32(46(16-38(53)65-20-60-26(2)48)17-39(54)66-21-61-27(3)49)35(13-25)58-11-12-59-42-31-14-36(43-45-15-37(71-43)44(57)69-24-64-30(6)52)70-34(31)10-9-33(42)47(18-40(55)67-22-62-28(4)50)19-41(56)68-23-63-29(5)51/h7-10,13-15H,11-12,16-24H2,1-6H3
• InChI Key: UTBYGIDPBQOHNT-UHFFFAOYSA-N

詳細

詳細

Cell permeant ester form of FURA 2 (Cat. No. 344900). The active dye is rapidly released in the cell by cytoplasmic esterases. Fluorescent calcium chelator (K_d = 224 nM) that offers 30 times greater fluorescence intensity than QUIN 2 (Cat. No. 551826). Can be used as a probe in many cellular systems, including cardiac myocytes, vascular smooth muscle cells, and medullary collecting tubules. Spectral properties in the presence of low calcium concentrations (excitation max.: 362 nm, emission max.: 512 nm) differ from those during high calcium conditions (excitation max.: 335 nm, emission max.: 505 nm). Note: 1 set = 10 x 100 µg.

Cell-permeable ester form of FURA 2. Note: 1 set = 10 x 100 µg.

PLURONIC^® F-127, PROTEIN GRADE Detergent (Cat. No. 540025, available separately) is a mild, low-toxicity detergent that can aid in the solubilization of FURA 2/AM.

生物化学的／生理学的作用

Cell permeable: yes

Primary Target
Ratiometric calcium indicator

Product does not compete with ATP.

Reversible: no

包装

Packaged under inert gas

警告

Toxicity: Standard Handling (A)

調製ノート

Further dilute with aqueous buffers just prior to use.

再構成

Following reconstitution, aliquot and freeze (-20°C) under an inert gas. Stock solutions are stable for up to 1 month at -20°C.

その他情報

Davis, M.J., et al. 1992. Am. J. Physiol. 263, H1292.
Rao, G.H., et al. 1992. Biochem. Med. Metab. Biol.47, 226.
Schwartz, Y., et al. 1992. Endocrinology131, 772.
Ward, C.A., and Moffat, M.P. 1992. J. Mol. Cell. Cardiol.24, 937.
Janconi, M.E., et al. 1990. J. Cell Biol.110, 1555.
Tsien, R.Y. 1980. Biochemistry19, 2396.

法的情報

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Pluronic is a registered trademark of BASF

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable