おすすめの製品
詳細
EHS Matrix Extract is a solubilized basement membrane preparation extracted from the Engelbreth-Holm-Swarm (EHS) mouse sarcoma. Its major components are laminin, collagen type IV, plaminogen activator and other growth factors which occur naturally in the EHS tumor.
アプリケーション
EHS Matrix Extract will gel rapidly at 22°C to 37°C. Thaw the EHS Matrix Extract at 4°C overnight in a refrigerator. Use pre-chilled pipettes, plates and tubes when preparing matrigel. Gelled EHS Matrix Extract may be liquefied by placing in refrigerator at 4°C overnight.
調製ノート
Take the appropriate culture medium for your cells from the refrigerator. Decontaminate the bottle with 70% alcohol in a sterile hood.
*Keep EHS Matrix Extract on ice throughout the preparation. Use chilled pipette tips, pipettes, culture plates/flasks and tubes for preparing EHS Matrix Extract.
For example:
2.5 ml for each T-25 flask (1.25 ml of culture medium to 1.25 ml of EHS Matrix Extract)
210 μl for each 24-well plate well (105 μL of culture medium to 105 μL of EHS Matrix Extract)
*Keep EHS Matrix Extract on ice throughout the preparation. Use chilled pipette tips, pipettes, culture plates/flasks and tubes for preparing EHS Matrix Extract.
- Thaw EHS Matrix Extract at 4°C overnight on ice.
- Use cooled pipette tips, mix the EHS Matrix Extract on ice to homogeneity.
- Prepare EHS Matrix Extract matrix by adding chilled culture medium to EHS Matrix Extract on ice in a 1:1 ratio.
- Coat tissue culture surface evenly.
For example:
2.5 ml for each T-25 flask (1.25 ml of culture medium to 1.25 ml of EHS Matrix Extract)
210 μl for each 24-well plate well (105 μL of culture medium to 105 μL of EHS Matrix Extract)
- Incubate at 37°C for one hour.
- Rinse EHS Matrix Extract with culture medium.
- EHS Matrix Extract coated tissue culture surface is now ready for use.
保管分類コード
10 - Combustible liquids
WGK
WGK 3
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
126-2.5:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
この製品を見ている人はこちらもチェック
Lital Livni et al.
Neuroscience letters, 694, 14-19 (2018-11-16)
Chemotherapy-induced peripheral neuropathy (CIPN) is a severe and debilitating adverse effect of cancer therapy that results from treatment with neurotoxic agents. Although chemotherapy treatment has been shown to inhibit neurite outgrowth from dorsal root ganglion (DRG) neurons in vitro, evidence
Zhen Yang et al.
Autophagy, 15(4), 668-685 (2018-11-06)
Emerging evidence has revealed that miRNAs could upregulate the expression levels of target genes. However, the molecular mechanism underlying upregulation of targets mediated by miRNAs remains unclear. In this study, we found a novel miRNA named MIR-G-1 by GRSF1-RNA immunoprecipitation
Richard Beatson et al.
Communications biology, 3(1), 644-644 (2020-11-06)
The tumour microenvironment plays a crucial role in the growth and progression of cancer, and the presence of tumour-associated macrophages (TAMs) is associated with poor prognosis. Recent studies have demonstrated that TAMs display transcriptomic, phenotypic, functional and geographical diversity. Here
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
製品に関するお問い合わせはこちら（テクニカルサービス）