07689

オメーラ試薬

suitable for microbiology

• 別名: フォーゲス・プロスカウエル反応用オメーラ試薬
• UNSPSCコード: 41171621
• NACRES: NA.85

特性

• 製品種目: BioChemika
• 品質水準: 100
• シェルフライフ: limited shelf life, expiry date on the label
• 組成: creatine, 0.3 g ; dist. water, 100 mL ; potassium hydroxide, 40 g
• 包装: pkg of 100 mL
• 保管条件: (Tightly closed. Dry.)
• テクニック: microbe id | metabolite detection: suitable
• アプリケーション: environmental; food and beverages; microbiology
• 適合性: Enterobacter spp.; Klebsiella spp.; Neisseria spp.; Pseudomonas spp.

詳細

詳細

O′Meara′s Reagent is used in the Voges-Proskauer (VP) test for the detection of acetoin production by bacterial strains. VP test is helpful in identifying members of the family Enterobacteriaceae based on their ability to produce acetylmethylcarbinol (AMC = acetonin) from glucose. Acetoin is an intermediate which is oxidized by oxygen under alkaline conditions (KOH) to 2,3-Butanediol. With creatinine (break down product from creatine) and the enhancement of α-naphtol a red color is formed (Meisenheimer complex)

アプリケーション

The O′Meara reagent is a component of the Voges-Proskauer (VP) test, which is a commonly used laboratory technique in industrial microbiology to differentiate between bacterial strains based on their ability to produce acetylmethylcarbinol (AMC) from glucose. The VP test is often used as a tool to identify and authenticate bacterial strains in the food, beverage, and pharmaceutical industries.

アナリシスノート

To perform the VP test, a bacterial sample is inoculated into a nutrient broth and incubated. The O′Meara reagent, which contains a mixture of creatinine and potassium hydroxide (KOH), is then added to the culture. Acetoin produced in the medium by bacterial culture is oxidized under alkaline conditions in the presence of air to form diacetyl which reacts with creatine to give a pinkish-red compound.

安全性情報

• ピクトグラム: GHS05,GHS07
• シグナルワード: Danger
• 危険有害性情報: H290,H302,H314
• 注意書き: P234 - P270 - P280 - P301 + P312 - P303 + P361 + P353 - P305 + P351 + P338
• 危険有害性の分類: Acute Tox. 4 Oral - Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1A
• 保管分類コード: 8B - Non-combustible corrosive hazardous materials
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable
• 個人用保護具 (PPE): Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter