1.09293

KOVACS′ indole reagent

for detecting microbial indole in the identification of indole-positive and indole-negative microorganisms, suitable for microbiology

• 別名: 4-(Dimethylamino)benzaldehyde solution, Indole reagent, Kovac′s reagent, Kovac′s reagent for indoles
• UNSPSCコード: 41106212
• NACRES: NA.21

特性

• 品質水準: 200
• フォーム: liquid
• 包装: pkg of 100 mL
• テクニック: microbe id | specific enzyme detection: suitable
• 色: yellow
• pH: <1 (20 °C in H₂O)
• 転移温度: flash point 36 °C
• 密度: 0.92 g/cm³ at 20 °C
• アプリケーション: microbiology
• 保管温度: 2-8°C
• 適合性: Escherichia coli; enterovirulent E. coli (EEC) spp.

詳細

詳細

Kovac′s reagent is a biochemical reagent composed of n-Butanol, hydrochloric acid, and 4-dimethylaminobenzaldehyde. It is used for the detection of indole producing microorganisms. Tryptophan is abundant in trypticalle digested peptone. Some microorganisms can cleave tryptophan to give pyruvic acid, ammonia, and indole. Indole then reacts with 4-dimethylaminobenzaldehyde to form a dark red dye. As tryptophan also gives a color reaction with 4 dimethylaminobenzaldehyde, it must be separated from the indole. This is achieved by selectively extracting indole with butanol.

アプリケーション

Kovac′s Indole reagent is used for the detection of indole producing microorganisms in food and beverages, and environmental samples. This is suitable for the identification of E. coli.

保管および安定性

No metal containers. Keep the container tightly closed in a dry and well-ventilated place. Keep away from heat and sources of ignition

アナリシスノート

Indole formation (Escherichia coli ATCC 25922 (WDCM 00013)): +
Indole formation (Proteus vulgaris ATCC 8427): +
Indole formation (Enterobacter aerogenes ATCC 13048): -
Tested in DEV-Tryptophan broth art. no. 1.10694. (after incubation 24 hrs.; 35 °C aerobic).

その他情報

The reagent solution must be stored in the dark in the refrigerator, otherwise it may turn brown and cannot be used.

安全性情報

• ピクトグラム: GHS02,GHS05,GHS07
• シグナルワード: Danger
• 危険有害性情報: H226 - H290 - H302 - H314 - H317 - H335 - H336
• 注意書き: P210 - P233 - P280 - P301 + P312 - P303 + P361 + P353 - P305 + P351 + P338
• 危険有害性の分類: Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Skin Corr. 1 - Skin Sens. 1
• 保管分類コード: 3 - Flammable liquids
• WGK: WGK 1
• 引火点(°F): 96.8 °F
• 引火点(℃): 36 °C