1.11350

Bactident^™ Kovac′s Indole reagent

Available in a practical dropper bottle, for Escherichia coli

• 別名: 4-(Dimethylamino)benzaldehyde solution, Indole reagent according to Kovac, Indole reagent, Indole test reagent
• UNSPSCコード: 41106200
• NACRES: NA.84

特性

• 品質水準: 100
• 詳細: Bactident™ E. coli 1.13303
• 形状: liquid (ready-to-use)
• シェルフライフ: 210 days
• 特徴: ready-to-use
• 組成: (n-Butanol; Hydrochloric acid; 4 dimethylaminobenzaldehyde)
• 包装: pkg of (1 x 30 ml, Plastic bottle)
• テクニック: microbe id | specific enzyme detection: suitable
• pH: <1 (20 °C in H₂O)
• 転移温度: flash point 36 °C
• 密度: 0.92 g/cm³ at 20 °C
• アプリケーション: environmental; food and beverages
• 保管温度: 2-8°C
• 適合性: Enterobacter spp.; Escherichia coli

詳細

詳細

Kovac′s reagent is a biochemical reagent composed of n-Butanol, hydrochloric acid, and 4-dimethylaminobenzaldehyde. It is used for the detection of indole producing microorganisms. Tryptophan is abundant in trypticalle digested peptone. Some microorganisms can cleave tryptophan to give pyruvic acid, ammonia, and indole. Indole then reacts with 4-dimethylaminobenzaldehyde to form a dark red dye. As tryptophan also gives a color reaction with 4 dimethylaminobenzaldehyde, it must be separated from the indole. This is achieved by selectively extracting indole with butanol.

アプリケーション

Bactident^™ Kovac′s Indole reagent is used for the detection of indole producing microorganisms in food and beverages, and environmental samples. This is suitable for the identification of E. coli.

関連事項

Replaces: 1.11350.0007

アナリシスノート

Indole formation
- Escherichia coli ATCC 25922 (WDCM 00013):
+
- Proteus vulgaris ATCC 8427: +
- Enterobacter aerogenes ATCC 13048: -
Incubation: 24 hrs.; 35 °C; aerobic.
Reference: DEV-Tryptophan broth.

法的情報

BACTIDENT is a trademark of Merck KGaA, Darmstadt, Germany

安全性情報

• ピクトグラム: GHS02,GHS05,GHS07
• シグナルワード: Danger
• 危険有害性情報: H226 - H290 - H302 - H314 - H317 - H335 - H336
• 注意書き: P210 - P233 - P280 - P301 + P312 - P303 + P361 + P353 - P305 + P351 + P338
• 危険有害性の分類: Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Skin Corr. 1 - Skin Sens. 1
• 保管分類コード: 3 - Flammable liquids
• WGK: WGK 1
• 引火点(°F): 96.8 °F
• 引火点(℃): 36 °C