RLI1MAG-92K

MILLIPLEX^® Rat Liver Injury Panel - Toxicity Multiplex Assay

• 別名: Luminex^® Rat Liver Toxicity Assay, Millipore Rat Liver Toxicity Assay, Rat Liver Injury Multiplex Panel
• UNSPSCコード: 12161503
• eCl@ss: 32161000
• NACRES: NA.84

特性

• 詳細: The analytes available for this multiplex kit are: Liver-type arginase 1 (ARG1), aspartate transaminase 1 (GOT1), α-glutathianone S-transferase (GSTα), sorbitol dehydrogenase (SDH), and 5′-Nucleotidase (5′-NT/CD73).
• 品質水準: 200
• 化学種の反応性: rat
• メーカー／製品名: Milliplex^®
• assay range: accuracy: 77-92%; sensitivity: 0.021-4.038 ng/mL; (MinDC+2SD); standard curve range: 0.02-15.0 ng/mL; (GSTα); standard curve range: 0.04-30.0 ng/mL; (5′-NT/CD73); standard curve range: 0.08-60.0 ng/mL; (GOT1); standard curve range: 0.10-75.0 ng/mL; (ARG1); standard curve range: 0.62-450.0 ng/mL; (SDH)
• テクニック: multiplexing: suitable
• 検出方法: fluorometric (Luminex xMAP)
• 輸送温度: wet ice

詳細

詳細

The MILLIPLEX^® Rat Liver Injury Bead Panel (RLI1MAG-92K) contains all the components necessary to simultaneous quantify the following 5 analytes in serum and plasma samples: Liver-Type Arginase 1 (ARG1)*, Aspartate transaminase 1 (GOT1), α-glutathione S-transferase (GSTα)*, Sorbitol Dehydrogenase (SDH)*, 5′-Nucleotidase/CD73 (5′-NT).

*ARG1, GSTα, and SDH are biomarkers listed in the Predictive Safety Testing Consortium (PSTC) project pipeline which have a strong translational role in drug safety testing.

The MILLIPLEX^® portfolio provides a valuable research assay to investigate multiple biomarkers of liver injury in rat serum and plasma samples using the Luminex^® xMAP^® instrument platform. This kit uses a 96-well format, contains a lyophilized standard cocktail, two quality controls and can measure up to 38 serum or plasma samples in duplicate.

Drug toxicity is the leading cause of acute liver failure in the United States. Patients with liver damage generally display elevated amounts of specific liver proteins in serum; these proteins can serve as biomarkers of drug-related liver toxicity. Monitoring these biomarkers can greatly help clinicians avoid drug-induced liver failure. Performing laboratory tests to characterize the side effects of potential therapeutics is an essential part of drug development. The rat is a leading animal model for these pre-clinical toxicity studies. The search for sensitive, organ-specific toxicity biomarkers is complemented by the development of novel assays to measure these critical analytes. Liver-Type Arginase 1 (ARG1), α-glutathione S-transferase (GSTα), and Sorbitol Dehydrogenase (SDH) are biomarkers listed in the Predictive Safety Testing Consortium (PSTC) project pipeline which have a strong translational role in drug safety testing. Aspartate transaminase 1 (GOT1) and 5′-Nucleotidase / CD73 (5′-NT) are traditional biomarkers recognized by both the Food and Drug Administration (FDA) and its European counterpart, the European Medicines Agency (EMA).

Panel Type: Toxicity

アプリケーション

• Analytes: 5′-Nucleotidase (5′-NT/CD73), Liver-Type Arginase 1 (ARG1), Aspartate transaminase 1 (GOT1), α-Glutathione S-Transferase (GSTα), Sorbitol Dehydrogenase (SDH)
• Recommended Sample Type: Rat serum and plasma
• Recommended Sample Dilution: 25 μL per well of 1:25 diluted serum or plasma
• Assay Run Time: 2 hours at room temperature (20-25°C)
• Research Category: Toxicity

特徴および利点

Design your multiplex kit by choosing available analytes within this panel.

構成

Rat Liver Injury Standard, 1 vial, lyophilized

Rat Liver Injury Quality Control 1, 1 vial, lyophilized

Rat Liver Injury Quality Control 2, 1 vial, lyophilized

Assay Buffer, 1 bottle, 30 mL

Wash Buffer, 10X (0.05% Proclin), 2 bottles, 30 mL ea

Rat Liver Injury Detection Antibodies, 1 bottle, 3.2 mL

Streptavidin-Phycoerythrin, 1 bottle, 3.2 mL

Mixing Bottle, 1 bottle

Sample Plate, 96 well format, 1 plate

2 Foil Plate Sealers

その他情報

Please contact Technical Service for linearity of dilution.

Sensitivity: Please see kit protocol for individual assay sensitivities.

法的情報

Luminex is a registered trademark of Luminex Corp

MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

xMAP is a registered trademark of Luminex Corp

安全性情報

• ピクトグラム: GHS06,GHS09
• シグナルワード: Danger
• 危険有害性情報: H302 + H332,H311,H317,H319,H411
• 注意書き: P273 - P280 - P301 + P312 - P302 + P352 + P312 - P304 + P340 + P312 - P305 + P351 + P338
• 危険有害性の分類: Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Irrit. 2 - Skin Sens. 1
• 保管分類コード: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects