MABT1341

Anti-Lamin A/C Antibody, clone 4C11

clone 4C11, from mouse

• 別名: 70 kDa Lamin, Renal carcinoma antigen NY-REN-32
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: mouse
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: 4C11, monoclonal
• 交差性: human, monkey, Syrian hamster, rat, mouse
• 包装: antibody small pack of 25 μg
• テクニック: ChIP: suitable; immunofluorescence: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• アイソタイプ: IgG2aκ
• NCBIアクセッション番号: NP_001244303
• UniProtアクセッション番号: P02545
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... LMNA(4000)

詳細

詳細

Prelamin-A/C (UniProt: P02545) is encoded by the LMNA (also known as LMN1) gene (Gene ID: 4000) in human. It is cleaved into Lamin-A/C (also known as 70 kDa Lamin, Renal carcinoma antigen NY-REN-32). Lamins are components of the nuclear lamina that provide a framework for the nuclear envelope and may also interact with chromatin. Lamin A and C are present in equal amounts in the lamina of mammals. Plays an important role in nuclear assembly, chromatin organization, nuclear membrane and telomere dynamics. Lamin A is initially synthesized as prelamin A that undergoes several modifications in the carboxyl terminal region that allow incorporation of prelamin A into the nuclear envelope and its subsequent processing into the mature lamin A. Cleavage of 15 residues (aa 647-662) by ZMPSTE24/FACE1 generates the final protein product. Unlike mature lamin A, prelamin A accumulates as discrete and localized foci at the nuclear periphery. Prelamin-A/C can accelerate smooth muscle cell senescence. It can act to disrupt mitosis and induce DNA damage in vascular smooth muscle cells (VSMCs), leading to mitotic failure, genomic instability, and premature senescence. Mutations in LMNA gene are known to cause Emery-Dreifuss muscular dystrophy that is characterized by weakness and atrophy of muscle without involvement of the nervous system. Some mutations have also been linked to familial type of lipodystrophy characterized by the loss of subcutaneous adipose tissue in the lower parts of the body. (Ref.: Casasola, A., et al. (2016). Nucleus 7(1); 84-102).

特異性

Clone 4C11 detects Lamin A/C at the nuclear lamina in multiple species.

免疫原

Purified protein corresponding to the Ig-fold of human Lamin A/C.

アプリケーション

Research Category
細胞骨格

Anti-Lamin A/C, clone 4C11, Cat. No. MABT1341, is a highly specific mouse monoclonal antibody that targets Lamin A/C and has been tested for use in Immunofluorescence, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP), and Western Blotting.

Western Blotting Analysis: A representative lot detected Lamin A/C in Western Blotting applications (Gesson, K., et. al. (2016). Genome Res. 26(4):462-73; Roblek, M., et. al. (2010). PLoS One. 5(5):e10604).

Dot Blot Analysis: A representative lot detected Lamin A/C in Dot Blot applications (Roblek, M., et. al. (2010). PLoS One. 5(5):e10604).

Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected Lamin A/C in HeLa cells.

Western Blotting Analysis: A representative lot detected Lamin A/C in WB used to demonstrate high titration and excellent properties of clone 4C11 (Courtesy of Marie lang, M.D., Stefan Schuchner, Ph.D. and Egon Ogris, M.D., Medical University of Vienna, Austria).

Immunofluorescence Analysis: A representative lot detected the Lamin A/C species at the nuclear lamina of HeLa cells (Courtesy of Marie Lang, M.D., Stefan Schuchner, Ph.D. and Egon Ogris, M.D., Medical University of Vienna, Austria).

Immunoprecipitation Analysis: A representative lot detected Lamin A/C in WB used to demonstrate high titration and excellent properties of clone 4C11 (Courtesy of Marie Lang, M.D., Stefan Schuchner, Ph.D. and Egon Ogris, M.D., Medical University of Vienna, Austria).

Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected Lamin A/C in Chromatin Immunoprecipitation applications (Gesson, K., et. al. (2016). Genome Res. 26(4):462-73).

Immunofluorescence Analysis: A representative lot detected Lamin A/C in Immunofluorescence applications (Roblek, M., et. al. (2010). PLoS One. 5(5):e10604).

Immunoprecipitation Analysis: A representative lot immunoprecipitated Lamin A/C in Immunoprecipitation applications (Gesson, K., et. al. (2016). Genome Res. 26(4):462-73).

品質

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 0.2 µg/mL of this antibody detected Lamin A/C in HeLa cell lysate.

ターゲットの説明

74 and 65 kDa observed. 74.14 kDa and 65.14 kDa calculated for Lamin A and C, respectively. Uncharacterized bands may be observed in some lysate(s).

物理的形状

Protein G purified

Format: Purified

Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

保管および安定性

Stable for 1 year at 2-8°C from date of receipt.

その他情報

Concentration: Please refer to lot specific datasheet.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable