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Key Documents

安全性情報

MABF3174

Sigma-Aldrich

Anti-IL-17A Antibody, clone 6F9.2.12.1.5

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About This Item

UNSPSCコード:
12352203
NACRES:
NA.41

由来生物

mouse

品質水準

結合体

unconjugated

抗体製品の状態

purified antibody

抗体製品タイプ

primary antibodies

クローン

6F9.2.12.1.5, monoclonal

分子量

calculated mol wt 17.5 kDa
observed mol wt ~30 kDa

精製方法

using protein G

化学種の反応性

human

包装

antibody small pack of 100 μg

テクニック

immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable

アイソタイプ

IgG1κ

エピトープ配列

Unknown

タンパク質IDアクセッション番号

UniProtアクセッション番号

輸送温度

2-8°C

ターゲットの翻訳後修飾

unmodified

遺伝子情報

human ... IL17A(7314)

詳細

Interleukin-17A (UniProt: Q16552; also known as IL-17, IL-17A, Cytotoxic T-lymphocyte-associated antigen 8, CTLA-8) is encoded by the IL17A (also known as CTLA8, IL17) gene (Gene ID: 3605) in human. IL-17A is a proinflammatory cytokine secreted by activated CD4+ Th17 cells. It is synthesized with a signal peptide (aa 1-23) that is subsequently cleaved off to produce the mature protein. Its levels are up-regulated by IL23A-IL12B. IL-17 is reported to play an important role in multiple biological processes, including the induction of IL-6, IL-8, G-CSF, and PGE2 production in epithelial, endothelial or fibroblasts; the enhancement of surface expression of ICAM-1 in fibroblasts; activation of NF-kB and co-stimulation of T cell proliferation. It is also involved in antimicrobial host defense and maintenance of tissue integrity. Its actions are mediated via interaction with IL-17RA and IL-17RC heterodimeric receptor complex, triggering homotypic interaction of these receptors with (TRAF3 Interacting Protein 2 (TRAF3IP2) adapter. This leads to downstream TRAF6-mediated activation of NF-kB and MAP kinase pathways resulting in transcriptional activation of cytokines, chemokines, antimicrobial peptides, and matrix metalloproteinases. IL-17A also plays an important role in connecting T cell-mediated adaptive immunity and acute inflammatory response to destroy extracellular bacteria and fungi. In synergy with IL-17F, it is shown to mediate the production of antimicrobial b-defensins DEFB1, DEFB103A, and DEFB104A by mucosal epithelial cells, which limits the entry of microbes through the epithelial barriers. (Ref.: Boisson, B., et al. (2013). Immunity. 39(4); 676-686; Wright, J.F., et al. (2008). J. Immunol. 181(4); 2799-2805).

特異性

Clone 6F9.2.12.1.5 is a mouse monoclonal antibody that specifically detects human Interleukin-17A.

免疫原

A recombinant fragment corresponding to amino acids 19 to 155 of human IL-17A.

アプリケーション

Quality Control Testing

Evaluated by Western Blotting with recombinant human IL-17A.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected recombinant human IL-17A.

Tested Applications

Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected IL-17A in human tonsil tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-IL-17A, clone 6F9.2.12.1.5, Cat. No. MABF3174, is a mouse monoclonal antibody that detects IL-17A and is tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

物理的形状

Purified mouse monoclonal antibody IgG1 in PBS with 0.05% sodium azide.

保管および安定性

Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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保管分類コード

12 - Non Combustible Liquids

WGK

WGK 2


適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

Jan Code

MABF3174-100UG:
MABF3174-25UG:


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