MABF2812

Anti-CD137 Antibody, clone LOB12.0

• 別名: 4-1BB ligand receptor, T-cell antigen 4-1BB, Tumor necrosis factor receptor superfamily member 9
• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: rat
• 品質水準: 200
• 抗体製品の状態: purified antibody
• 抗体製品タイプ: primary antibodies
• クローン: LOB12.0, monoclonal
• 分子量: calculated mol wt 27.6 kDa; observed mol wt ~N/A kDa
• 精製方法: using protein G
• 化学種の反応性: mouse
• 包装: antibody small pack of 100
• テクニック: competitive inhibition ELISA: suitable; flow cytometry: suitable; inhibition assay: suitable; surface plasmon resonance (SPR): suitable
• アイソタイプ: IgG2aκ
• エピトープ配列: Unknown
• タンパク質IDアクセッション番号: NP_001070977
• UniProtアクセッション番号: P20334
• 保管温度: -10 to -25°C
• 遺伝子情報: mouse ... Tnfrsf9(21942)

詳細

特異性

Clone LOB12.0 is a rat monoclonal antibody that detects CD137 (Tnfrsf9).

免疫原

Mouse CD137 human Fc fusion protein

アプリケーション

Quality Control Testing

Isotype testing: Identity confirmation by Isotyping test.

Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be rat IgG2a .

Tested Applications

Surface plasmon resonance: A representative lot detected CD137 in Surface plasmon resonance applications (Buchan, S.L., et al. (2018). Immunity. 49(5):958-970.e.7).

Flow Cytometry Analysis: 10 µg from a representative lot detected CD137 in Jurkat cells expressing mouse CD137.

Agonist Activity: A representative lot of this antibody co-stimulated CD8+ T cells and potentiated anti-tumor immunity in mice.(Taraban, V.Y., et al. (2002). Eur J Immunol. 32(12); 3617-3627 ).

Competition Assay: A representative lot of this antibody competed for binding to 4-1BB on effector T cells. (Buchan, S.L., et al. (2018). Immunity. 49(5):958-970.e.7).

Flow Cytometry Analysis: A representative lot detected CD137 in Flow Cytometry applications (Buchan, S.L., et al. (2018). Immunity. 49(5):958-970.e.7).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

ターゲットの説明

Tumor necrosis factor receptor superfamily member 9 (UniProt: P20334; also known as 4-1BB ligand receptor, T-cell antigen 4-1BB, CD137) is encoded by the Tnfrsf9 (also known as Cd137, IIa, Ly63) gene (Gene ID: 21942) in murine species. CD137 is a single-pass type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-23), which is subsequently cleaved off to produce the mature protein that contains an extracellular domain (aa 24-187), a transmembrane domain (aa 187-208), and a cytoplasmic domain (aa 209-256). It is a member of the TNFR superfamily that serves as an activation-induced costimulatory molecule. Its expression is observed on activated T cells, NK cells, dendritic cells, eosinophils, mast cells, endothelial cells, and some tumor cells. It is rapidly expressed on activated CD8+ T cells and at lower levels on activated CD4+ T cells. Induction of CD137 expression on CD8+ T cells is reported to be highly sensitive to low levels of TCR stimulation. Engagement of CD137 on T cells stimulates their proliferation independently of CD28 and promotes IL-2 and IFN- secretion by Th effector cells and CTLs, respectively. Expression of CD137 is shown to negatively correlate with lymphocyte proliferation and positively with the degree of activation-induced cell death caused by mitogen overstimulation. Treg cells that infiltrate tumors are reported to express higher levels of CD137 and administration of clone LOB12.0 with agonistic function promotes T cell agonism to promote tumor rejection. (Ref.: Buchan, SL., et al. (2018). Immunity 49(5), 958-970; Taraban, VY., et al. (2002). Eur. J. Immunol. 32(12); 3617-3627).

物理的形状

Purified rat monoclonal antibody IgG2a in PBS without preservatives.

再構成

0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

保管および安定性

Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable