MABE132

Anti-MyoD Antibody, clone 5F11

clone 5F11, from rat

• 別名: Myoblast determination protein 1
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: rat
• 品質水準: 100
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: 5F11, monoclonal
• 化学種の反応性: mouse
• テクニック: immunocytochemistry: suitable; western blot: suitable
• アイソタイプ: IgG2aκ
• NCBIアクセッション番号: NP_034996
• UniProtアクセッション番号: P10085
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: mouse ... Myod1(17927)

詳細

詳細

Myoblast determination protein 1 (MyoD1) is a myogenic transcription factor required for the growth, repair, and regeneration of muscle cells. It induces differentiation of myoblast cells into multinucleated myotubules, activating genes and proteins involved in cell cycle arrest, and differentiation. MyoD1 may also control proliferation and homeostasis during embryogenesis, in the absence of Myf5.

免疫原

GST-tagged recombinant protein corresponding to mouse MyoD.

アプリケーション

Research Category
細胞シグナル伝達

Research Sub Category
発生シグナル伝達

Detect MyoD using this Anti-MyoD Antibody, clone 5F11 validated for use in Western Blotting, ICC.

Immunocytochemistry Analysis: A representative lot detected MyoD in C2C12 cells.

Western Blot Analysis: A representative lot detected MyoD in C2C12 cells.

品質

Evaluated by Western Blot in C2C12 cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected MyoD in 10 µg of C2C12 cell lysate.

ターゲットの説明

~45 kDa observed. An uncharacterized band at ~38 kDa may be observed in some cell lysates. The calculated molecular weight is 34 kDa; however, this protein is known to be glycosylated.

関連事項

Replaces: MABE514

物理的形状

Protein G Purified

Format: Purified

Purified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

保管および安定性

Stable for 1 year at 2-8°C from date of receipt.

アナリシスノート

Control
C2C12 cell lysate

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable