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Merck
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Key Documents

安全性情報

MABN707

Sigma-Aldrich

Anti-DCX Antibody, clone 2G5

clone 2G5, from mouse

別名:

Neuronal migration protein doublecortin, Doublin, Lissencephalin-X, Lis-X, DCX

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About This Item

UNSPSCコード:
12352203
eCl@ss:
32160702
NACRES:
NA.41

由来生物

mouse

品質水準

抗体製品の状態

purified antibody

抗体製品タイプ

primary antibodies

クローン

2G5, monoclonal

化学種の反応性

mouse, human

テクニック

flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

アイソタイプ

IgG1

UniProtアクセッション番号

輸送温度

wet ice

ターゲットの翻訳後修飾

unmodified

遺伝子情報

mouse ... Dcx(13193)

詳細

Doublecortin (DCX) or alternatively known as Doublin or Lissencephalin-X (Lis-X) and encoded by the human gene named DCX/DBCN/LISX is a microtubule associated protein that is required for the initial steps of neuronal dispersion and lamination during cortex brain development. Expressed by neuronal precursor cells and immature neurons, DCX expression is decreased by the time neurons are mature and express the mature neuronal marker NeuN. Thus DCX expression is a hallmark of developing neurons and neurogenesis. DCX expression is localized in the cytoplasm and along microtubule tracks. DCX appears to stabilize microtubules and causes bundling of them in the cytoplasm. In disease, DCX is the cause for an X-linked lissencephaly and subcortical laminar heterotopia, a disorder that is characterized by a “smooth” brain with few folds like normal brains and poorly formed cortical layers as well as other abnormalities. Besides developmental effects DCX may potentially help in the suppression of gliomas because in brain tumor stem cells (BTSC) self-renewal ability was significantly reduced in cells expressing DCX compared to control cells. EMD-Millipore’s Anti-DCX mouse monoclonal has been tested in western blot against recombinant protein as well as mouse heart lysate. The antibody has also been successfully tested in paraffin embedded immunohistochemistry on normal human brain and kidney cancer tissue sections as well as in fluorescent immunocytochemistry on HepG2 cells in culture and flow cytometry on SK-N-SH cells in culture and direct ELISA on varying concentrations of antigen.

免疫原

Purified recombinant fragment of human DCX expressed in E. Coli.

アプリケーション

Research Category
ニューロサイエンス
Research Sub Category
ニューロン発生
Detect Doublecortin using this mouse monoclonal antibody, Anti-DCX Antibody, clone 2G5 validated for use in western blotting, IHC, ICC & Flow Cytometry.
Immunohistochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected DCX in human brain and kidney cancer tissues.

Immunofluorescence Analysis: A 1:200-1,000 dilution from a representative lot detected DCX in HepG2 cells.

Flow Cytometry Analysis: A 1:200-400 dilution from a representative lot detected DCX in non serum starved SK-N-SH cells.

Optimal working dilutions must be determined by end user.

品質

Evaluated by Western Blotting in Mouse heart lysate.

Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected DCX in mouse heart lysate.

ターゲットの説明

~50 kDa observed. Uncharacterized bands may appear in some lysate(s).

物理的形状

Protein G Purified
Format: Purified
Purified mouse monoclonal in PBS with up to 0.1% sodium azide and 0.5% protein stabilizer.

保管および安定性

Stable for 1 year at 2-8°C from date of receipt.

アナリシスノート

Control
Mouse heart lysate

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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保管分類コード

12 - Non Combustible Liquids

WGK

WGK 2

引火点(°F)

Not applicable

引火点(℃)

Not applicable


適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

Jan Code

MABN707:


試験成績書(COA)

製品のロット番号・バッチ番号を入力して、試験成績書(COA) を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。

以前この製品を購入いただいたことがある場合

文書ライブラリで、最近購入した製品の文書を検索できます。

文書ライブラリにアクセスする

Vincent Quoc-Huy Trinh et al.
The Journal of pathology, 258(1), 69-82 (2022-06-11)
The development of neural structures within tumors is now considered vital for carcinogenesis. However, the time course of this development in human pre-invasive neoplasia has been incompletely described. Therefore, we performed a detailed analysis of nerves across the neoplastic spectrum
Christopher T Rhodes et al.
Cell cycle (Georgetown, Tex.), 17(3), 377-389 (2018-02-13)
Histone methyltransferases (HMTs) are present in heterogeneous cell populations within the adult brain including neurogenic niches. Yet the question remains whether loss of HMTs and the resulting changes in histone methylation alter cell fate in a region-specific manner. We utilized
Wenshu Zhou et al.
Stem cell research & therapy, 12(1), 174-174 (2021-03-14)
Spinal cord injury (SCI) is a debilitating medical condition that can result in the irreversible loss of sensorimotor function. Current therapies fail to provide an effective recovery being crucial to develop more effective approaches. Mesenchymal stem cell (MSC) exosomes have
Michael R Foret et al.
Frontiers in genetics, 5, 252-252 (2014-08-16)
Histone 3 Lysine 9 (H3K9) methylation is known to be associated with pericentric heterochromatin and important in genomic stability. In this study, we show that trimethylation at H3K9 (H3K9me3) is enriched in an adult neural stem cell niche- the subventricular
Richard S Sandstrom et al.
Scientific reports, 4, 5371-5371 (2014-06-21)
Histone 3 lysine 4 trimethylation (H3K4me3) is known to be associated with transcriptionally active or poised genes and required for postnatal neurogenesis within the subventricular zone (SVZ) in the rodent model. Previous comparisons have shown significant correlation between baboon (Papio

ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.

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