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由来生物
rat
品質水準
抗体製品の状態
purified immunoglobulin
抗体製品タイプ
primary antibodies
クローン
3F3A, monoclonal
化学種の反応性
mouse, human
テクニック
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
アイソタイプ
IgMκ
NCBIアクセッション番号
UniProtアクセッション番号
輸送温度
ambient
ターゲットの翻訳後修飾
unmodified
遺伝子情報
human ... GPR78(27201)
詳細
E3 ubiquitin-protein ligase AMFR (UniProt Q9UKV5; also known as AMF receptor, Autocrine motility factor receptor, gp78, RING finger protein 45) is encoded by the RNF45 (also known as AMFR) gene (Gene ID 267) in human. Glycoprotein 78 (gp78) is an endoplasmic reticulum (ER) membrane-anchored E3 ubiquitin ligase that plays a key role in ER-associated degradation (ERAD). Gp78 is localized to a mitochondria-associated ER domain, where it induces mitochondrial fragmentation by targeting the mitochondrial fusion proteins mitofusin 1 and 2 (Mfn1 and Mfn2, respectively) for degradation upon mitochondrial depolarization. Other known ERAD substrates processed by gp78 include ApoB lipoprotein, HMG CoA reductase, CD3-delta, cystic fibrosis transmembrane conductance regulator, the metastasis suppressor KAI1, unglycosylated prion protein (PrP), and the T-cell receptor. Gp78 is a 7-transmembrane protein (a.a. 82-102, 122-142, 145-165, 186-206, 215-235, 276-296, 429-449) with a RING-type Zinc finger (a.a. 341-379), a CUE domain (a.a. 456-498), and a VCP/p97-interacting motif (VIM; a.a. 622-640).
特異性
Clone 3F3A detected glycoprotein 78 (gp78) and a ~150 kDa glycoprotein in B16-F1 mouse melanoma cell extract. Cell extract sialidase treatment decreased the antibody′s immunoreactivity toward gp78 and abolished ~150 kDa band detection (Nabi, I.R., et al. (1990). Cancer Res. 50(2):409-414).
免疫原
Epitope: extracellular domain
Peanut lectin (PNA) resin-purified membrane glycoprotein 78 from B16-F1 cells (Nabi, I.R., and Raz, A. (1987). Int. J. Cancer. 40(3):396-402).
アプリケーション
Research Category
アポトーシス及び癌
アポトーシス及び癌
Detect GP78 using this rat monoclonal Anti-Glycoprotein 78, clone 3F3A, Cat. No. MABC949, validated for use in Function Assays, Immunocytochemistry, Immunohistochemistry, and Western Blotting.
Immunocytochemistry: A representative lot detected glycoprotein 78 immunoreactivity colocalized with that of the mitochondria marker OxPhosV in COS-7 cells (Courtesy of I. Robert Nabi, Ph.D., University of British Columbia, Canada).
Affects Function: A representative lot stimulated the mobility of B16-F1 mouse melanoma cells on colloidal gold-coated surface (Nabi, I.R., et al. (1990). Cancer Res. 50(2):409-414).
Immunocytochemistry Analysis: A representative lot detected both surface and cytoplasmic gp78 immunoreactivity by fluorescent immunocytochemistry staining of intact or MeOH/3% paraformaldehyde-fixed and 0.5% Triton X-100-permeablized mouse A31 fibroblasts and A31M angiosarcoma cells (Niinaka, Y., et al. (1998). Cancer Res. 58(12):2667-2674).
Immunocytochemistry Analysis: Clone 3F3A ascites fluid immunolocalized gp78 on the surface of adherent B16-F1 mouse melanoma cells by fluorescent immunocytochemistry staining of 3% paraformaldehyde-fixed cells (Nabi, I.R., et al. (1990). Cancer Res. 50(2):409-414).
Immunohistochemistry Analysis: A representative lot detected an elevated gp78 immunoreactivity in frozen mammary gland sections from 235RLF transgenic mice than age-matched wild type mice (Joshi, B., et al. (2010). J. Biol. Chem. 285(12):8830-8839).
Western Blotting Analysis: A representative lot detected shRNA-mediated gp78 downregulation in HT-1180 human fibrosarcoma cells (Fu, M., et al. (2013). Mol. Biol. Cell. 24(8):1153-1162).
Western Blotting Analysis: A representative lot detected endogenous gp78 and gp78 transgene expression in mammary glands from wild-type and transgenic mice. Clone 3F3A detected a higher gp78 expression in metastatic MDA-435 cells than in non-metastatic MCF7 breast carcinoma cells (Joshi, B., et al. (2010). J. Biol. Chem. 285(12):8830-8839).
Western Blotting Analysis: A representative lot detected much higher gp78 expression in angiosarcoma (human HT-1180 and mouse A31M) than in fibroblast (human IMR90 and mouse A31) lines (Niinaka, Y., et al. (1998). Cancer Res. 58(12):2667-2674).
Western Blotting Analysis: A representative lot detected gp78 and a ~150 kDa band in B16-F1 mouse melanoma cell extract. Autocrine motility factor (AMF) competed against clone 3F3A for binding the gp78 target band. Cell extract sialidase treatment decreased the antibody′s immunoreactivity toward gp78 and abolished ~150 kDa band detection (Nabi, I.R., et al. (1990). Cancer Res. 50(2):409-414).
Affects Function: A representative lot stimulated the mobility of B16-F1 mouse melanoma cells on colloidal gold-coated surface (Nabi, I.R., et al. (1990). Cancer Res. 50(2):409-414).
Immunocytochemistry Analysis: A representative lot detected both surface and cytoplasmic gp78 immunoreactivity by fluorescent immunocytochemistry staining of intact or MeOH/3% paraformaldehyde-fixed and 0.5% Triton X-100-permeablized mouse A31 fibroblasts and A31M angiosarcoma cells (Niinaka, Y., et al. (1998). Cancer Res. 58(12):2667-2674).
Immunocytochemistry Analysis: Clone 3F3A ascites fluid immunolocalized gp78 on the surface of adherent B16-F1 mouse melanoma cells by fluorescent immunocytochemistry staining of 3% paraformaldehyde-fixed cells (Nabi, I.R., et al. (1990). Cancer Res. 50(2):409-414).
Immunohistochemistry Analysis: A representative lot detected an elevated gp78 immunoreactivity in frozen mammary gland sections from 235RLF transgenic mice than age-matched wild type mice (Joshi, B., et al. (2010). J. Biol. Chem. 285(12):8830-8839).
Western Blotting Analysis: A representative lot detected shRNA-mediated gp78 downregulation in HT-1180 human fibrosarcoma cells (Fu, M., et al. (2013). Mol. Biol. Cell. 24(8):1153-1162).
Western Blotting Analysis: A representative lot detected endogenous gp78 and gp78 transgene expression in mammary glands from wild-type and transgenic mice. Clone 3F3A detected a higher gp78 expression in metastatic MDA-435 cells than in non-metastatic MCF7 breast carcinoma cells (Joshi, B., et al. (2010). J. Biol. Chem. 285(12):8830-8839).
Western Blotting Analysis: A representative lot detected much higher gp78 expression in angiosarcoma (human HT-1180 and mouse A31M) than in fibroblast (human IMR90 and mouse A31) lines (Niinaka, Y., et al. (1998). Cancer Res. 58(12):2667-2674).
Western Blotting Analysis: A representative lot detected gp78 and a ~150 kDa band in B16-F1 mouse melanoma cell extract. Autocrine motility factor (AMF) competed against clone 3F3A for binding the gp78 target band. Cell extract sialidase treatment decreased the antibody′s immunoreactivity toward gp78 and abolished ~150 kDa band detection (Nabi, I.R., et al. (1990). Cancer Res. 50(2):409-414).
品質
Western Blotting Analysis: A 1:62.5 dilution of this antibody detected glycoprotein 78 (gp78) in 50 µg of HeLa cell lysate.
ターゲットの説明
~78 kDa observed. Target band size appears larger than the calculated molecular weight of 73.00 kDa due to glycosylation. Uncharacterized bands may be observed in some lysate(s).
物理的形状
Format: Purified
Purified rat IgM in buffer containing PBS without azide.
保管および安定性
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
その他情報
Concentration: Please refer to lot specific datasheet.
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
12 - Non Combustible Liquids
WGK
WGK 2
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
MABC949:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
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