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由来生物
mouse
品質水準
抗体製品の状態
ascites fluid
抗体製品タイプ
primary antibodies
クローン
B10, monoclonal
化学種の反応性
rat, human
メーカー／製品名
Chemicon®
テクニック
ELISA: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable
アイソタイプ
IgG2a
NCBIアクセッション番号
UniProtアクセッション番号
輸送温度
wet ice
ターゲットの翻訳後修飾
unmodified
遺伝子情報
human ... HSPG2(3339)
特異性
The peptide used as the immunogen for this product correspond to the amino-acid sequence 151-165 from human ER alpha receptor. By IP, western, gel retardation and ELISA assays clone B10 do not react with other nuclear receptors.
SPECIES REACTIVITIES:
Does not cross react with chicken ER. Other reactivities have not been evaluated. By sequence homology pig ER is 100% identical, rat is 80% conserved.
SPECIES REACTIVITIES:
Does not cross react with chicken ER. Other reactivities have not been evaluated. By sequence homology pig ER is 100% identical, rat is 80% conserved.
免疫原
Epitope: a.a. 151-165
アプリケーション
Research Category
エピジェネティクス及び核内機能分子
エピジェネティクス及び核内機能分子
Research Sub Category
転写因子
転写因子
Immunohistochemistry (frozen sections, microwave oven treated paraffin sections) at 1:1,000-1:2,000
Immunocytochemistry (formaldehyde fixation) at 1:1,000-1:2,000
ELISA at 1:1,000-1:2,000
Western blot analysis at 1:1,000-1:2,000
Immunoprecipitation at 1:1-1:10
Gel super shifts at 1:1-1:10
Optimal working dilutions must be determined by the end user.
Immunocytochemistry (formaldehyde fixation) at 1:1,000-1:2,000
ELISA at 1:1,000-1:2,000
Western blot analysis at 1:1,000-1:2,000
Immunoprecipitation at 1:1-1:10
Gel super shifts at 1:1-1:10
Optimal working dilutions must be determined by the end user.
Use Anti-Estrogen Receptor Antibody, clone B10 (Mouse Monoclonal Antibody) validated in ELISA, WB, IHC(P) to detect Estrogen Receptor also known as ER.
物理的形状
Ascites. Liquid, does not contain any preservative.
保管および安定性
Maintain at -20°C in undiluted aliquots up to 12 months. Avoid repeated freeze/thaw cycles.
法的情報
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
10 - Combustible liquids
WGK
WGK 1
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
MAB463:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Evidence for a potential role of estrogen in the penis: detection of estrogen receptor-alpha and -beta messenger ribonucleic acid and protein.
Subrina Jesmin, Chishimba N Mowa, Naoyuki Matsuda, Alaa-Eldin Salah-Eldin, Hiroko Togashi et al.
Endocrinology null
John G Geisler et al.
Diabetes, 51(7), 2158-2169 (2002-06-28)
Type 2 diabetes is characterized by loss of beta-cell mass and concomitant deposition of amyloid derived from islet amyloid polypeptide (IAPP). Previously we have shown that expression of human IAPP (huIAPP) in islets of transgenic mice results in either a
P Bhat-Nakshatri et al.
British journal of cancer, 90(4), 853-859 (2004-02-19)
Oestrogen receptor alpha (ERalpha) is an oestrogen-activated transcription factor, which regulates proliferation and differentiation of mammary epithelial cells by activating or repressing gene expression. ERalpha is a critical prognostic indicator and a therapeutic target for breast cancer. Patients with tumours
The poly(A)-dependent transcriptional pause is mediated by CPSF acting on the body of the polymerase.
Anita Nag,Kazim Narsinh,Harold G Martinson
Nature Structural and Molecular Biology null
Estrogen receptor beta expression in invasive breast cancer.
S Mann, R Laucirica, N Carlson, P S Younes, N Ali, A Younes, Y Li, M Younes
Human Pathology null
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