MAB353B

Anti-Nestin Antibody, clone rat-401, Biotin Conjugate

clone rat-401, from mouse, biotin conjugate

• 別名: rat401, NES, Nbla00170, FLJ21841
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: mouse
• 品質水準: 100
• 結合体: biotin conjugate
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: rat-401, monoclonal
• 交差性: mouse, rat
• 以下との反応禁止: human
• テクニック: immunocytochemistry: suitable; immunohistochemistry: suitable
• アイソタイプ: IgG1
• NCBIアクセッション番号: NP_037119
• UniProtアクセッション番号: P21263
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... NES(10763) ; mouse ... Nes(18008) ; rat ... Nes(25491)

詳細

詳細

Nestin, a large intermediate filament protein (class Type VI) expressed during development and in myotendinous and neuromuscular junctions. Nestin expression is restricted, typically disappearing by E18. Nestin is thought to be a reasonable neuronal marker; however, recent studies have found nestin expression in other cell types such as endothelial cells (Mokry and Nemecek, 1998). Nestin identifies the most primitive neuroepithelium but also identifies many other embryonic tissues, so it is not specific for CNS. Nestin expression is seen in almost all GBMs (Glioblastoma mulitformes) and many melanomas (both primary and metastatic) but not in any metastatic carcinomas.

特異性

This antibody recognizes both rat and mouse Nestin. Antibody is not reactive with human Nestin protein.

免疫原

Embryonic rat spinal cord

アプリケーション

Research Category
幹細胞研究

ニューロサイエンス

Research Sub Category
ニューロン発生

神経幹細胞

Detect Nestin using this mouse monoclonal antibody, Anti-Nestin Antibody, clone rat-401, Biotin Conjugate validated for use in ICC & IHC.

Evaluated by Immunocytochemistry in rat hippocampal neural stem cells (SCR022).
Immunocytochemsitry Analysis: A 1:200 dilution of this antibody detected Nestin in rat hippocampal neural stem cells (SCR022).
Evaluated by Immunocytochemistry in ReNcell VM human neural progenitor cells (SCC008).
Immunocytochemsitry Analysis: A 1:200 dilution of this antibody did not detect Nestin in ReNcell VM human neural progenitor cells (SCC008). Evaluated by Immunohistochemistry in rat hindbrain brain tissue.
Immunohistochemsitry Analysis: A 1:50 dilution of this antibody detected Nestin in rat hindbrain brain tissue.

品質

Evaluated by Immunocytochemistry in mouse cortical neural stem cells (SCR029). Immunocytochemsitry Analysis: A 1:200 dilution of this antibody detected Nestin in mouse cortical neural stem cells (SCR029).

ターゲットの説明

200-220 kDa calculated

物理的形状

Protein G Purified

Purified mouse monoclonal IgG1 conjugated to Biotin in PBS with 0.1% sodium azide and 15mg/ml BSA.

保管および安定性

Maintain refrigerated at 2-8ºC protected from light in undiluted aliquots for up to 6 months from date of receipt.

アナリシスノート

Control
Mouse cortical neural stem cells (SCR029)

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable