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由来生物
mouse
品質水準
抗体製品の状態
purified antibody
抗体製品タイプ
primary antibodies
クローン
rat-401, monoclonal
化学種の反応性
mouse, rat
以下との反応禁止
human
メーカー／製品名
Chemicon®
テクニック
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
入力
sample type neural stem cell(s)
sample type epithelial cells
アイソタイプ
IgG1
NCBIアクセッション番号
UniProtアクセッション番号
輸送温度
wet ice
ターゲットの翻訳後修飾
unmodified
遺伝子情報
human ... NES(10763)
mouse ... Nes(18008)
rat ... Nes(25491)
詳細
Nestin, a large intermediate filament protein (class Type VI) expressed during development and in myotendinous and neuromuscular junctions. Nestin expression is restricted, typically disappearing by E18. Nestin is thought to be a reasonable neuronal marker; however, recent studies have found nestin expression in other cell types such as endothelial cells (Mokry and Nemecek, 1998). Nestin identifies the most primitive neuroepithelium but also identifies many other embryonic tissues, so it is not specific for CNS. Nestin expression is seen in almost all GBMs (Glioblastoma mulitformes) and many melanomas (both primary and metastatic) but not in any metastatic carcinomas.
特異性
Rodent Nestin protein confirmed with rat and mouse. Rat-401 is not reactive with human nestin protein.
免疫原
Nestin purified from embryonic rat spinal cord.
アプリケーション
Research Category
幹細胞研究
幹細胞研究
Research Sub Category
神経幹細胞
神経幹細胞
Detect Nestin using this Anti-Nestin Antibody, clone rat-401 validated for use in IC, IH, IH(P) & WB.
Western Blot:
200-220 kDa in reducing gels of newborn rat or mouse cell extracts. Note that nestin was originally reported (erroneously) to migrate at approximately 440 kDa by SDS-PAGE (Kachinsky, 1995).
For Westerns, prepare samples by boiling in 4 volumes of 125 mM Tris, pH 6.8, 10% 2-mercaptoethanol, 10% glycerol and 4.6% SDS. 5% PAGE gels are suggested. Block membranes with milk or BSA. Do not dilute primary antibody in buffer containing milk. Freezing and thawing of samples or samples not stored at -85°C will show breakdown of nestin sometimes into multiple fragments.
Immunohistochemistry:
1:20-1:200 dilution of a previous lot was used. Perfuse tissue with 4% paraformaldehyde at pH 7.4 for light microscopy or with either 4% paraformaldehyde at pH 10.0 or 4% paraformaldehyde with 0.1% glutaraldehyde at pH 7.4 for EM. Suitable for frozen or paraffin-embedded sections.
Immunocytochemistry:
1:20-1:200 dilution of a previous lot was used. Fix cells in 4% paraformaldehyde buffered with 50 mM sodium borate at pH 9.5.
Optimal working dilutions and protocols must be determined by end user.
200-220 kDa in reducing gels of newborn rat or mouse cell extracts. Note that nestin was originally reported (erroneously) to migrate at approximately 440 kDa by SDS-PAGE (Kachinsky, 1995).
For Westerns, prepare samples by boiling in 4 volumes of 125 mM Tris, pH 6.8, 10% 2-mercaptoethanol, 10% glycerol and 4.6% SDS. 5% PAGE gels are suggested. Block membranes with milk or BSA. Do not dilute primary antibody in buffer containing milk. Freezing and thawing of samples or samples not stored at -85°C will show breakdown of nestin sometimes into multiple fragments.
Immunohistochemistry:
1:20-1:200 dilution of a previous lot was used. Perfuse tissue with 4% paraformaldehyde at pH 7.4 for light microscopy or with either 4% paraformaldehyde at pH 10.0 or 4% paraformaldehyde with 0.1% glutaraldehyde at pH 7.4 for EM. Suitable for frozen or paraffin-embedded sections.
Immunocytochemistry:
1:20-1:200 dilution of a previous lot was used. Fix cells in 4% paraformaldehyde buffered with 50 mM sodium borate at pH 9.5.
Optimal working dilutions and protocols must be determined by end user.
品質
Immunohistochemistry(paraffin):
Nestin (cat. # MAB353, Clone 401) staining of Mouse Pancreas, tissue was pretreated with citrate buffer, pH 6.0. A previous lot of this antibody was diluted to 1.25 µg/mL, using IHC-Select detection with HRP-DAB. Immunoreactivity is detected in the islets of Langerhans.
Optimal Staining of Nestin Monoclonal Antibody: Mouse Pancreas
Nestin (cat. # MAB353, Clone 401) staining of Mouse Pancreas, tissue was pretreated with citrate buffer, pH 6.0. A previous lot of this antibody was diluted to 1.25 µg/mL, using IHC-Select detection with HRP-DAB. Immunoreactivity is detected in the islets of Langerhans.
Optimal Staining of Nestin Monoclonal Antibody: Mouse Pancreas
ターゲットの説明
200-220 kDa
物理的形状
Protein A purified
Format: Purified
Purified mouse monoclonal IgG1 in buffer containing 0.09% sodium azide.
保管および安定性
Stable for 1 year at 2-8ºC from date of receipt.
アナリシスノート
Control
Mouse embryonic brain tissue
Mouse embryonic brain tissue
その他情報
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法的情報
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
12 - Non Combustible Liquids
WGK
WGK 1
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
MAB353:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
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資料
Human iPSC neural differentiation media and protocols used to generate neural stem cells, neurons and glial cell types.
プロトコル
Step-by-step culture protocols for neural stem cell culture including NSC isolation, expansion, differentiation and characterization.
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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