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由来生物
mouse
品質水準
抗体製品の状態
ascites fluid
抗体製品タイプ
primary antibodies
クローン
1MOD-1A9, monoclonal
交差性
mouse, human
メーカー／製品名
Chemicon®
テクニック
ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
アイソタイプ
IgG1
NCBIアクセッション番号
UniProtアクセッション番号
輸送温度
dry ice
ターゲットの翻訳後修飾
unmodified
遺伝子情報
human ... CBX1(10951)
詳細
Chromatin assembly factor-1 (CAF-1) is a multisubunit protein complex that comprises three polypeptide subunits known as p150, p60, and p48. CAF-1 is a nucleosome assembly factor that deposits newly synthesized and acetylated histones H3/H4 into nascent chromatin during DNA replication.
Heterochromatin is characterized as densely coiled chromatin that generally replicates late during S phase, has a low gene density, and contains large blocks of repetitive DNA that is relatively inaccessible to DNA-modifying reagents. In late S phase, p150 directly associates with heterochromatin associated proteins 1 (HP1α, HP1β and HP1γ). As cells prepare for mitosis, CAF-1 p150 and some HP1 progressively dissociate from heterochromatin, coinciding with the phosphorylation of histone H3. The HP1 proteins reassociate with chromatin at the end of mitosis, as histone H3 is dephosphorylated.
Heterochromatin is characterized as densely coiled chromatin that generally replicates late during S phase, has a low gene density, and contains large blocks of repetitive DNA that is relatively inaccessible to DNA-modifying reagents. In late S phase, p150 directly associates with heterochromatin associated proteins 1 (HP1α, HP1β and HP1γ). As cells prepare for mitosis, CAF-1 p150 and some HP1 progressively dissociate from heterochromatin, coinciding with the phosphorylation of histone H3. The HP1 proteins reassociate with chromatin at the end of mitosis, as histone H3 is dephosphorylated.
特異性
Reacts with Heterochromatin protein-1 b (HP1b).
免疫原
Recombinant mouse HP1beta.
アプリケーション
Use Anti-Heterochromatin Protein-1 β Antibody, clone 1MOD-1A9 (Mouse Monoclonal Antibody) validated in ELISA, ICC, IHC, IP, WB to detect Heterochromatin Protein-1 β also known as HP1b.
Western blot: 1:500-1:5,000
Immunofluorescence: 1:500-1:5,000
Immunohistochemistry: 1:500-1:5,000
Immunoprecipitation: 1:500 - 1:5,000
ELISA: 1:500-1:5,000
Optimal working dilutions must be determined by the end user.
Immunofluorescence: 1:500-1:5,000
Immunohistochemistry: 1:500-1:5,000
Immunoprecipitation: 1:500 - 1:5,000
ELISA: 1:500-1:5,000
Optimal working dilutions must be determined by the end user.
ターゲットの説明
28 kDa
アナリシスノート
Control
HeLa, 293 or NIH/3T3 cell lysates
HeLa, 293 or NIH/3T3 cell lysates
その他情報
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法的情報
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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保管分類コード
10 - Combustible liquids
WGK
WGK 1
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
MAB3448:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Structural differences in centromeric heterochromatin are spatially reconciled on fertilisation in the mouse zygote.
Aline V Probst, Fatima Santos, Wolf Reik, Genevieve Almouzni, Wendy Dean
Chromosoma null
The chemokine growth-regulated oncogene 1 (Gro-1) links RAS signaling to the senescence of stromal fibroblasts and ovarian tumorigenesis.
Yang, G; Rosen, DG; Zhang, Z; Bast, RC; Mills, GB; Colacino, JA; Mercado-Uribe, I; Liu, J
Proceedings of the National Academy of Sciences of the USA null
Epigenetic regulation of myogenic gene expression by heterochromatin protein 1 alpha.
Sdek, P; Oyama, K; Angelis, E; Chan, SS; Schenke-Layland, K; MacLellan, WR
Testing null
Jmjd1a and Jmjd2c histone H3 Lys 9 demethylases regulate self-renewal in embryonic stem cells.
Loh, YH; Zhang, W; Chen, X; George, J; Ng, HH
Genes & Development null
Essential role of DPPA3 for chromatin condensation in mouse oocytogenesis.
Liu, YJ; Nakamura, T; Nakano, T
Biology of reproduction null
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