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Merck

MAB331-C

Sigma-Aldrich

Anti-SNAP-25 Antibody, clone SP14 (Ascites Free)

clone SP14, from mouse

別名:

Synaptosomal-associated protein 25, SNAP-25, Super protein, SUP, Synaptosomal-associated 25 kDa protein, SNAP-25

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About This Item

UNSPSCコード:
12352203
eCl@ss:
32160702
NACRES:
NA.41

由来生物

mouse

品質水準

抗体製品の状態

purified immunoglobulin

抗体製品タイプ

primary antibodies

クローン

SP14, monoclonal

交差性

monkey, bovine, mouse, rat, guinea pig, human

テクニック

activity assay: suitable
electron microscopy: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable

アイソタイプ

IgG1κ

NCBIアクセッション番号

UniProtアクセッション番号

ターゲットの翻訳後修飾

unmodified

遺伝子情報

human ... SNAP25(6616)

詳細

Synaptosomal-associated protein 25 (UniProt P60880; also known as SNAP-25, SUP, Super protein, Synaptosomal-associated 25 kDa protein) is encoded by the SNAP25 (also known as SNAP) gene (Gene ID 6616) in human. SNAP-25 belongs to the superfamily of soluble N-ethylmaleimide-sensitive factor (NSF) attachment protein (SNAP) receptor (SNARE) proteins involved in mediating vesicle fusion events. Target or t-SNAREs located in the membranes of target compartments form stable subcomplexes and engage vesicle or v-SNAREs in the membranes of transport vesicles to facilitate the fusion. SNAP-25 is a t-SNARE protein of the trans-SNARE complex that executes fusion between the synaptic vesicle and plasma membrane. SANP-25 does not have a transmembrane domain and is anchored to the cytosolic face of membranes via palmitoyl side chains covalently linked to cysteine residues.

特異性

Clone SP41 recognizes both SNAP-25 isoforms A & B. Clone SP41 is reactive toward endogenous or exogenously expressed SNAP-25 in COS cells, but not SNAP-25 expressed in E. coli, indicative of a posttranslational modification-dependent antigenicity.

免疫原

Epitope: N-terminal half
Human brain proteins immunoprecipitated with EP10 (Cat. No. MAB332).

アプリケーション

Research Category
ニューロサイエンス
Research Sub Category
ニューロン発生
Anti-SNAP-25 Antibody, clone SP14 (Ascites Free) is an antibody against SNAP-25 for use in Western Blotting, Immunohistochemistry (Paraffin), Electron Microscopy and Activity Assay.

品質

Evaluated by Western Blotting in human brain tissue lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected SNAP-25 in 10 µg of human brain tissue lysate.

ターゲットの説明

~25 kDa observed

関連事項

Replaces: MAB331

物理的形状

Protein G Purified
Format: Purified
Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl without preservatives.

保管および安定性

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

その他情報

Concentration: Please refer to lot specific datasheet.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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保管分類コード

12 - Non Combustible Liquids

WGK

WGK 1

引火点(°F)

Not applicable

引火点(℃)

Not applicable


試験成績書(COA)

製品のロット番号・バッチ番号を入力して、試験成績書(COA) を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。

以前この製品を購入いただいたことがある場合

文書ライブラリで、最近購入した製品の文書を検索できます。

文書ライブラリにアクセスする

Calpain activity contributes to the control of SNAP-25 levels in neurons.
Grumelli, C; Berghuis, P; Pozzi, D; Caleo, M; Antonucci, F; Bonanno, G; Carmignoto et al.
Molecular and Cellular Neurosciences null
Most peptide-containing sensory neurons lack proteins for exocytotic release and vesicular transport of glutamate.
Morris, JL; Konig, P; Shimizu, T; Jobling, P; Gibbins, IL
The Journal of Comparative Neurology null
Measuring synaptosomal associated protein-25 kDa in human cerebral spinal fluid.
Thompson, PM; Rosenberger, C; Holt, S; Perrone-Bizzozero, NI
Journal of Psychiatric Research null
Heterogeneous expression of SNAP-25 in rat and human brain.
Garbelli, R; Inverardi, F; Medici, V; Amadeo, A; Verderio, C; Matteoli, M; Frassoni, C
The Journal of Comparative Neurology null
Autoregulation in PC12 cells via P2Y receptors: Evidence for non-exocytotic nucleotide release from neuroendocrine cells.
Hussl, S; Kubista, H; Boehm, S
Purinergic Signaling null

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