AB5585-I

Anti-Recoverin Antibody

from rabbit, purified by affinity chromatography

• 別名: Cancer-associated retinopathy protein, Cancer-associated retinopathy antigen, Protein CAR, Recoverin
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: rabbit
• 品質水準: 100
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 精製方法: affinity chromatography
• 化学種の反応性: human, rat
• 化学種の反応性（ホモロジーによる予測）: mouse (based on 100% sequence homology)
• テクニック: immunohistochemistry: suitable; western blot: suitable
• NCBIアクセッション番号: NP_002894
• UniProtアクセッション番号: P35243
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: mouse ... Rcvrn(19674)

詳細

詳細

Recoverin (UniProt P35243; also known as Cancer-associated retinopathy antigen, Cancer-associated retinopathy protein, Protein CAR) is encoded by the RCVRN (also known as RCV1) gene (Gene ID 5957) in human. The photoreceptor protein recoverin is a member of the EF-hand neuronal calcium sensor (NCS) proteins. Recoverin is initially produced as a 200-amino acid protein whose N-terminal initiator methionine is removed posttranslationally. Recoverin is composed of four EF-hand domains (a.a. 25-60, 61-96, 97-132, 147-182), with the second and third EF-hand regions mediating low- and high-affinity calcium binding, respectively. In photoreceptor cells, recoverin functions as a Ca2+-dependent inhibitor of G-protein-coupled receptor kinase 1 (GRK1). Recoverin plays an important role in mediating calcium-dependent rod and cone light adaptation. Recoverin delays the rod & cone response under dim light when [Ca2+] is high by inhibiting GRK1-catalyzed visual pigment phosphorylation, but has little effect during light adaptation when [Ca2+]i is lowered.

免疫原

Epitope: EF-hand 4

KLH-conjugated linear peptide corresponding to a sequence from the EF-hand 4 domain of human recoverin.

アプリケーション

Research Category
ニューロサイエンス

Research Sub Category
感覚・末梢神経

Anti-Recoverin Antibody detects level of Recoverin and has been published and validated for use in Western Blotting, Immunohistochemistry.

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Recoverin in human and rat retina tissue sections.

品質

Evaluated by Western Blotting in human retina tissue lysate.

Western Blotting Analysis: 0.25 µg/mL of this antibody detected Recoverin in 10 µg of human retina tissue lysate.

ターゲットの説明

~23 kDa observed.

物理的形状

Affinity purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

保管および安定性

Stable for 1 year at 2-8°C from date of receipt.

その他情報

Concentration: Please refer to lot specific datasheet.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable