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由来生物
mouse
品質水準
抗体製品の状態
purified antibody
抗体製品タイプ
primary antibodies
クローン
SP15, monoclonal
交差性
human, monkey, feline, rat, mouse
テクニック
ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
アイソタイプ
IgMκ
NCBIアクセッション番号
UniProtアクセッション番号
ターゲットの翻訳後修飾
unmodified
遺伝子情報
human ... SYP(6855)
詳細
Synaptophysin (UniProt P08247; also known as Major synaptic vesicle protein p38) is encoded by the SYP (also known as MRX96, MRXSYP) gene (Gene ID 6855) in human. Synaptophysin is a 38 kDa four-transmembrane glycoprotein on synaptic vesicle (SV) with both its N- and C-termini exposed cytoplasmically. Synaptophysin forms homomultimers in the SV membrane and is heavily phosphorylated on tyrosine residues in its C-terminal pentapeptide repeats. Synaptophysin is also known to interact with SV v-SNARE protein synaptobrevin II (sybII) when sybII is not engaged with other SNARE proteins, suggesting a role of synaptophysin in sybII retrieval during SV endocytosis. Consistently, synaptophysin-knockout neurons display reduced levels of sybII at their nerve terminals.
免疫原
Human brain proteins immunoprecipitated with EP10 (Cat. No. MAB332).
アプリケーション
Research Category
ニューロサイエンス
ニューロサイエンス
Research Sub Category
ニューロン発生
ニューロン発生
This Anti-Synaptophysin Antibody, clone SP15 (Ascites Free) is validated for use in Western Blotting, Immunohistochemistry, Immunocytochemistry, ELISA for the detection of Synaptophysin.
Western Blotting Analysis: 1.0 µg/mL from a representative lot detected Synaptophysin in 10 µg of human brain tissue lysate.
Western Blotting Analysis: A representative lot detected synaptophysin in rat hippocampal tissue extracts (Lin, D., et al. (2012). Behav. Brain Res. 228(2):319-327).
Western Blotting Analysis: Representative lots detected synaptophysin in mouse brain synaptosomes preparations (Shim, S.Y., et al. (2008). J. Neurosci. 28(14):3604-3614).
Western Blotting Analysis: A representative lot detected synaptophysin in the large dense-core vesicles-containing fractions obtained by sucrose gradient centrifugation of rat brain median eminence (ME) neuroterminals preparations (Yin, W., et al. (2007). Exp. Biol. Med. (Maywood). 232(5):662-673).
Immunohistochemistry Analysis: A representative lot detected a stronger synaptophysin immunoreactivity in the inner molecular layer of frozen dentate gyrus sections from a 3-week old monkey, while a stronger immunoreactivity was seen in the outer molecular layer of dentate gyrus sections from a 13-year old monkey (Lavenex, P., et al. (2007). Dev. Neurosci. 29(1-2):179–192).
Immunohistochemistry Analysis: A representative lot immunostained nerve terminals at palisade ending using cat extraocular muscle (EOM) whole mount sections (Konakci, K.Z., et al. (2005). Invest. Ophthalmol. Vis. Sci. 46(1):155-165).
Immunohistochemistry Analysis: A representative lot detected synaptophysin immunoreactivity in human brain tissue sections (Honer, W.G., et al. (1993). Brain Res. 609(1-2):9-20).
Immunocytochemistry Analysis: A representative lot detected punctate synatophysin immunoreactivity among cultured primary cerebrocortical neurons from rat embryos (Bragina, L., et al. (2006). J. Neurochem. 99(1):134-141.).
ELISA Analysis: Representative lots were employed as the capture antibody for the detection of synaptophysin in human temporal cortex, frontal cortex, and cerebellar cortex extracts by sandwich ELISA (Klucken, J., et al. (2006). Acta Neuropathol. 111(2):101-108; Fukumoto, H., et al. (2002). Arch. Neurol. 59(9):1381-1389).
Western Blotting Analysis: A representative lot detected synaptophysin in rat hippocampal tissue extracts (Lin, D., et al. (2012). Behav. Brain Res. 228(2):319-327).
Western Blotting Analysis: Representative lots detected synaptophysin in mouse brain synaptosomes preparations (Shim, S.Y., et al. (2008). J. Neurosci. 28(14):3604-3614).
Western Blotting Analysis: A representative lot detected synaptophysin in the large dense-core vesicles-containing fractions obtained by sucrose gradient centrifugation of rat brain median eminence (ME) neuroterminals preparations (Yin, W., et al. (2007). Exp. Biol. Med. (Maywood). 232(5):662-673).
Immunohistochemistry Analysis: A representative lot detected a stronger synaptophysin immunoreactivity in the inner molecular layer of frozen dentate gyrus sections from a 3-week old monkey, while a stronger immunoreactivity was seen in the outer molecular layer of dentate gyrus sections from a 13-year old monkey (Lavenex, P., et al. (2007). Dev. Neurosci. 29(1-2):179–192).
Immunohistochemistry Analysis: A representative lot immunostained nerve terminals at palisade ending using cat extraocular muscle (EOM) whole mount sections (Konakci, K.Z., et al. (2005). Invest. Ophthalmol. Vis. Sci. 46(1):155-165).
Immunohistochemistry Analysis: A representative lot detected synaptophysin immunoreactivity in human brain tissue sections (Honer, W.G., et al. (1993). Brain Res. 609(1-2):9-20).
Immunocytochemistry Analysis: A representative lot detected punctate synatophysin immunoreactivity among cultured primary cerebrocortical neurons from rat embryos (Bragina, L., et al. (2006). J. Neurochem. 99(1):134-141.).
ELISA Analysis: Representative lots were employed as the capture antibody for the detection of synaptophysin in human temporal cortex, frontal cortex, and cerebellar cortex extracts by sandwich ELISA (Klucken, J., et al. (2006). Acta Neuropathol. 111(2):101-108; Fukumoto, H., et al. (2002). Arch. Neurol. 59(9):1381-1389).
品質
Evaluated by Western Blotting in rat brain tissue lysate.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected Synaptophysin in 10 µg of rat brain tissue lysate.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected Synaptophysin in 10 µg of rat brain tissue lysate.
ターゲットの説明
~34 kDa observed
物理的形状
Format: Purified
Purified mouse monoclonal IgMκ antibody in PBS with 0.05% sodium azide.
保管および安定性
Stable for 1 year at 2-8°C from date of receipt.
その他情報
Concentration: Please refer to lot specific datasheet.
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
12 - Non Combustible Liquids
WGK
WGK 2
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
MAB329-C:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Beta-secretase protein and activity are increased in the neocortex in Alzheimer disease.
Fukumoto, H; Cheung, BS; Hyman, BT; Irizarry, MC
Archives of Neurology null
GLT-1 down-regulation induced by clozapine in rat frontal cortex is associated with synaptophysin up-regulation.
Bragina, L; Melone, M; Fattorini, G; Torres-Ramos, M; Vallejo-Illarramendi, A; Matute, C; Conti, F
Journal of Neurochemistry null
Clinical and biochemical correlates of insoluble alpha-synuclein in dementia with Lewy bodies.
Klucken, J; Ingelsson, M; Shin, Y; Irizarry, MC; Hedley-Whyte, ET; Frosch, M; Growdon et al.
Acta neuropathologica null
Molecular characteristics suggest an effector function of palisade endings in extraocular muscles.
Konakci, KZ; Streicher, J; Hoetzenecker, W; Blumer, MJ; Lukas, JR; Blumer, R
Investigative Ophthalmology & Visual Science null
Postnatal development of the primate hippocampal formation.
Lavenex, P; Banta Lavenex, P; Amaral, DG
Developmental Neuroscience null
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