MAB1059

Anti-Pigment Epithelium Derived Factor Antibody, clone 10F12.2

clone 10F12.2, Chemicon^®, from mouse

• 別名: pigment epithelium-derived factor, proliferation-inducing protein 35, serine (or cysteine) proteinase inhibitor, clade F (alpha-2 ; ; antiplasmin, pigment epithelium derived factor), member 1, serpin peptidase inhibitor, clade F (alpha-2 antiplasmin
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: mouse
• 品質水準: 100
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: 10F12.2, monoclonal
• 化学種の反応性: human
• 化学種の反応性（ホモロジーによる予測）: rat
• メーカー／製品名: Chemicon^®
• テクニック: ELISA: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• アイソタイプ: IgG2a
• NCBIアクセッション番号: NM_002615.4
• UniProtアクセッション番号: P36955
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... SERPINF1(5176)

詳細

詳細

Pigment epithelium-derived factor (PEDF) was first identified as a 50 kDa secreted protein in conditioned medium from cultured fetal human retinal pigment epithelium (RPE) cells (Tombran-Tink, 1991). More recently, PEDF was shown to have neurotrophic activity in the retina in vivo (Cayouette, 1999). PEDF is also a neurotrophic and neuroprotective factor in other systems, including cultured cerebellar granule cells, primary hippocampal neurons, spinal cord motor neurons and amphibian photoreceptors cultured in the absence of RPE cells (Karakousis, 2001). The binding of PEDF to retinoblastoma and cerebellar granule cells may be receptor-mediated. Recently, PEDF was shown to be a potent inhibitor of angiogenesis in endothelial cell cultures and in rat cornea and retina in vivo (Dawson, 1999; Stellmach, 2001).

特異性

Recognizes Pigment-Epithelium Derived Factor (PEDF).

免疫原

Recombinant human GST-PEDF fusion protein. The sequence corresponding to amino
acids 196-347 of human PEDF tagged with GST on the N-terminal was expressed in E. Coli. GST-PEDF is about 46 kDa on SDS-PAGE.

アプリケーション

Research Category
ニューロサイエンス

Research Sub Category
神経化学・ニューロトロフィン

Anti-Pigment Epithelium Derived Factor Antibody, clone 10F12.2 is an antibody against Pigment Epithelium Derived Factor for use in ELISA, IC, IH & WB.

Western Blot:
Recognizes a protein with a molecular weight of ~46 kDa when tested against immunogen. Western blot positive control is available (Catalog number AG672)

ELISA:
A previous lot of this antibody was used on ELISA.

Immunocytochemistry:
A previous lot of this antibody was used on Immunocytochemistry.

Immunohistochemistry:
A previous lot of this antibody was used on frozen sections.

Optimal working dilutions must be determined by end user.

品質

Routinely evaluated by Western Blot on human placenta lysate.

Western Blot Analysis:
1:1000 dilution of this lot detected pigment epithelium on 10 μg of human placenta lysate.

ターゲットの説明

~46 kDa

物理的形状

Protein A purified

Format: Purified

Purified mouse monoclonal IgG2a in buffer containing 0.02 M PBS, pH 7.6, 0.25 M NaCl with 0.1% sodium azide.

保管および安定性

Stable for 6 months at 2-8°C in undiluted aliquots from date of receipt.

アナリシスノート

Control
Eye tissue.

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法的情報

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable