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由来生物
mouse
品質水準
抗体製品の状態
purified antibody
抗体製品タイプ
primary antibodies
クローン
P5D2, monoclonal
化学種の反応性
human
メーカー／製品名
Chemicon®
テクニック
ELISA: suitable
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
アイソタイプ
IgG2bκ
UniProtアクセッション番号
輸送温度
wet ice
ターゲットの翻訳後修飾
unmodified
遺伝子情報
human ... ITGB1(3688)
詳細
Integrin beta 1, also known as CD29, is a 130 kDa transmembrane glycoprotein that forms noncovalent complexes with various Integrin alpha subunits (including alpha 1, alpha 2, alpha 3, alpha 4, alpha 5, and alpha 6) to form the functional receptors that bind to specific extracellular matrix proteins. Integrin receptors are involved in the regulation of a variety of important biological functions, including embryonic development, wound repair, hemostasis, and prevention of programmed cell death. They are also implicated in abnormal pathological states such as tumor directed angiogenesis, tumor cell growth, and metastasis. These heterodimeric receptors bridge the cytoplasmic actin cytoskeleton with proteins present in the extracellular matrix and/or on adjacent cells. Interactions between integrins and the extracellular matrix lead to activation of signal transduction pathways and regulation of gene expression.
特異性
Reacts with Human Integrin Beta 1.
免疫原
Human keratinocytes derived from skin.
アプリケーション
Research Category
細胞骨格
細胞骨格
Research Sub Category
インテグリン
インテグリン
Immunoprecipitation: A representative lot of this antibody clone was used in immunoprecipitation.
Immunohistochemistry: A representative lot of this antibody clone was used in immunohistochemistry (acetone fixation, no paraffin embedding).
ELISA: A representative lot of this antibody was used in ELISA.
Immunocytochemistry: A representative lot of this antibody clone was used in immunocytochemistry (paraformaldehyde fixation at less than 4%).
Functional Activity Assay: A representative lot of this antibody clone was used in cell attachment assay of SV-HFO cells with a characteristic spread morphology. In the presence of function-blocking mAbs to β1 integrin (P5D2), the cells attached but no longer spread, and displayed a rounded morphology with many cytoplasmic projections (Iba, K. et al., 2000).
Immunohistochemistry: A representative lot of this antibody clone was used in immunohistochemistry (acetone fixation, no paraffin embedding).
ELISA: A representative lot of this antibody was used in ELISA.
Immunocytochemistry: A representative lot of this antibody clone was used in immunocytochemistry (paraformaldehyde fixation at less than 4%).
Functional Activity Assay: A representative lot of this antibody clone was used in cell attachment assay of SV-HFO cells with a characteristic spread morphology. In the presence of function-blocking mAbs to β1 integrin (P5D2), the cells attached but no longer spread, and displayed a rounded morphology with many cytoplasmic projections (Iba, K. et al., 2000).
Anti-Integrin β1 Antibody, clone P5D2 (Azide Free) detects level of Integrin β1 & has been published & validated for use in ELISA, FC, IC, IH, IP, FUNC.
品質
Flow Cytometry Analysis:
4 µg of the antibody was used to detect Integrin β1 in 1x10^6 A431 cells.
4 µg of the antibody was used to detect Integrin β1 in 1x10^6 HeLa cells.
4 µg of the antibody was used to detect Integrin β1 in 1x10^6 A431 cells.
4 µg of the antibody was used to detect Integrin β1 in 1x10^6 HeLa cells.
物理的形状
Protein A purified
Format: Purified
Purified ascites in PBS without preservatives.
保管および安定性
Store at -20°C in undiluted aliquots.
Avoid repeated freeze-thaw cycles.
Avoid repeated freeze-thaw cycles.
アナリシスノート
Control
Human tonsil, human skin tissue
A431 & HeLa Cells
Human tonsil, human skin tissue
A431 & HeLa Cells
その他情報
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法的情報
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
12 - Non Combustible Liquids
WGK
WGK 2
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
MAB1959Z:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Pablo Castro-Córdova et al.
Nature communications, 12(1), 1140-1140 (2021-02-20)
Clostridioides difficile spores produced during infection are important for the recurrence of the disease. Here, we show that C. difficile spores gain entry into the intestinal mucosa via pathways dependent on host fibronectin-α5β1 and vitronectin-αvβ1. The exosporium protein BclA3, on
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