CBL221
Anti-RNA Polymerase II Antibody, clone ARNA-3
ascites fluid, clone ARNA-3, Chemicon®
別名:
DNA-directed RNA polymerase II subunit RPB1, DNA-directed RNA polymerase III largest subunit, RNA polymerase II subunit B1
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About This Item
UNSPSCコード:
12352203
eCl@ss:
32160702
NACRES:
NA.41
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由来生物
mouse
品質水準
抗体製品の状態
ascites fluid
抗体製品タイプ
primary antibodies
クローン
ARNA-3, monoclonal
化学種の反応性
kangaroo, human, Drosophila
メーカー／製品名
Chemicon®
テクニック
immunohistochemistry: suitable
western blot: suitable
アイソタイプ
IgG1
NCBIアクセッション番号
UniProtアクセッション番号
輸送温度
wet ice
ターゲットの翻訳後修飾
unmodified
遺伝子情報
human ... POLR2A(5430)
詳細
DNA-directed RNA polymerase II subunit RPB1 (EC 2.7.7.6; UniProt P04052; also known as DNA-directed RNA polymerase III largest subunit, RNA polymerase II subunit B1) is encoded by the RpII215 (also known as CG1554) gene (Gene ID 32100) in drosophila melanogaster (fruit fly). RNA polymerase II (Pol II) is a multi-subunit enzyme responsible for the transcription of protein-coding genes. Transcription initiation requires recruitment of the complete transcription machinery to a promoter via solicitation by activators and chromatin remodeling factors. Pol II can coordinate 10 to 14 subunits. This complex interacts with the promoter regions of genes and a variety of elements and transcription factors. The DNA binding domain of the polymerase is a groove where TFIIB orients the DNA for unwinding and transcription.
特異性
ARNA-3 is excellent for detection of transcription activity, e.g. puffs in polytene chromosomes. Reacts with RNA polymerase II in biological materials. Reactive polypeptides after SDS-PAGE: 200 kDa band of nonphosphorylated and 240 kDa band of phosphorylated Pol IIA.
Broad species cross-reactivity expected due to sequence homology.
免疫原
Epitope: Amino acids 794-822
Purified Drosophila RNA polymerase II
アプリケーション
Detect RNA Polymerase II with Anti-RNA Polymerase II Antibody, clone ARNA-3 (Mouse Monoclonal Antibody), that has been shown to work in WB & IHC.
Western blot
Immunohistochemistry: 1:100; Frozen tissue; fixation recommendation - incubate tissue with 2% paraformaldehyde in PBS for 10 min, wash in PBS for 5 min, incubate in 0.2% Triton X-100 in PBS for 5 min, wash in PBS for 5 min. Incubate with antibody one hour.
Optimal working dilutions must be determined by the end user.
Immunohistochemistry: 1:100; Frozen tissue; fixation recommendation - incubate tissue with 2% paraformaldehyde in PBS for 10 min, wash in PBS for 5 min, incubate in 0.2% Triton X-100 in PBS for 5 min, wash in PBS for 5 min. Incubate with antibody one hour.
Optimal working dilutions must be determined by the end user.
物理的形状
Supplied as hybridoma culture supernatant concentrate containing 0.09% sodium azide.
アナリシスノート
Control
Positive for drosophila, kangaroo kidney epithelial cell line PtK2, and human HeLa and HEp-2 cell lines.
Positive for drosophila, kangaroo kidney epithelial cell line PtK2, and human HeLa and HEp-2 cell lines.
法的情報
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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保管分類コード
10 - Combustible liquids
WGK
WGK 1
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
CBL221:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Tim Patrick Kaminski et al.
Molecular biology of the cell, 25(14), 2190-2198 (2014-05-23)
Regulation of RNA polymerase II (RNAPII) during transcription is essential for controlling gene expression. Here we report that the transcriptional activity of RNAPII at the Balbiani ring 2.1 gene could be halted during stable elongation in salivary gland cells of
Monoclonal antibody directed against RNA polymerase II of Drosophila melanogaster.
Kramer, A, et al.
Molecular & General Genetics, 180, 193-199 (1980)
Mapping of linear epitopes recognized by monoclonal antibodies with gene-fragment phage display libraries.
Petersen, G, et al.
Molecular & General Genetics, 249, 425-431 (1995)
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