ABS245

Anti-PKM2 Antibody

from rabbit, purified by affinity chromatography

• 別名: Embryonic enzyme pyruvate kinase M2, Pyruvate kinase isozymes M1/M2, Cytosolic thyroid hormone-binding protein, CTHBP, Opa-interacting protein 3, OIP-3, Pyruvate kinase 2/3, Pyruvate kinase muscle isozyme, Thyroid hormone-binding protein 1, THBP1, Tumor
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: rabbit
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 精製方法: affinity chromatography
• 交差性: monkey, human, mouse
• 交差性（ホモロジーによる予測）: rat (based on 100% sequence homology), bovine (based on 100% sequence homology), horse (based on 100% sequence homology)
• テクニック: immunocytochemistry: suitable; western blot: suitable
• NCBIアクセッション番号: NP_872270
• UniProtアクセッション番号: P14618
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... PKM(5315)

詳細

詳細

Pyruvate kinase isozymes M1/M2 (PKM2) is also called Cytosolic thyroid hormone-binding protein (CTHBP), Opa-interacting protein 3 (OIP-3), Pyruvate kinase 2/3, Pyruvate kinase muscle isozyme, Thyroid hormone-binding protein 1 (THBP1) and p58. PKM2 is a glycolytic enzyme that assists in generating ATP. PKM2 also stimulates POU5F1-mediated transcriptional activation, and plays a role in caspase independent cell death of tumor cells. The ratio between the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. This control of glycolysis and is important for tumor cell proliferation and survival. PKM2 is specifically expressed in proliferating cells, such as embryonic stem cells, embryonic carcinoma cells, and cancer cells.

特異性

This antibody recognizes PKM2 near the C-terminus.

免疫原

Epitope: Near C-terminus

KLH-conjugated linear peptide corresponding to human PKM2 near the C-terminus.

アプリケーション

Research Category
細胞シグナル伝達

Research Sub Category
代謝ホルモン及び受容体

Anti-PKM2 Antibody is a highly specific rabbit polyclonal antibody, that targets Pyruvate Kinase & has been tested in western blotting & ICC.

Immunocytochemistry Analysis: A 1:50 dilution from a representative lot detected PKM2 in HeLa cells.

品質

Evaluated by Western Blotting in P19 cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected PKM2 in 10 µg of P19 cell lysate.

ターゲットの説明

~60 kDa observed

物理的形状

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

保管および安定性

Stable for 1 year at 2-8°C from date of receipt.

アナリシスノート

Control
P19 cell lysate

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable