ABE40

Anti-PABPC1/C3 Antibody

from rabbit, purified by affinity chromatography

• 別名: Polyadenylate-binding protein 1, PABP-1, Poly(A)-binding protein 1
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: rabbit
• 品質水準: 100
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 精製方法: affinity chromatography
• 化学種の反応性: mouse, human
• 化学種の反応性（ホモロジーによる予測）: canine (based on 100% sequence homology), bovine (based on 100% sequence homology), chicken (based on 100% sequence homology), rat (based on 100% sequence homology), Xenopus (based on 100% sequence homology)
• テクニック: immunocytochemistry: suitable; western blot: suitable
• NCBIアクセッション番号: NP_002559
• UniProtアクセッション番号: P11940
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... PABPC1(26986)

詳細

詳細

Poly(A)-binding proteins, cytoplasmic 1 (PABPC1), and cytoplasmic 3 (PABPC3) are RNA-binding proteins with important roles in post-translational control and metabolism of mRNA. Although both proteins bind to the poly(A) tail of mRNA, PABPC3 binds with a lower affinity than PABPC1. Studies have associated these proteins with tumorigenesis. Decreased expression of PABPC1 in esophageal cancer seems to associate with tumor development and poor prognosis after surgery. PABPC1 can shuttle through the nuclear membrane and is thought to associate with newly synthesized poly(A) transcripts. PABPC1 has also been linked to microRNA mediated silencing when in contact with GW182. PABPC3 is thought to be specific to human testis and controls RNA stability.

免疫原

KLH-conjugated linear peptide corresponding to human PABPC1/C3. The immunogen sequence used is 100% identical to both PABPC1 and PABPC3.

アプリケーション

Research Category
エピジェネティクス及び核内機能分子

Research Sub Category
RNA代謝及び結合タンパク質

Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected PABPC1/C3 in NIH/3T3 and HeLa cells.

Use Anti-PABPC1/C3 (Rabbit Polyclonal Antibody) validated in ICC, WB to detect PABPC1/C3 also known as Polyadenylate-binding protein 1, PABP-1.

品質

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected PABPC1/C3 on 10 µg of HeLa cell lysate.

ターゲットの説明

~71 kDa observed

物理的形状

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

保管および安定性

Stable for 1 year at 2-8°C from date of receipt.

アナリシスノート

Control
HeLa cell lysate

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable