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由来生物
rabbit
品質水準
抗体製品の状態
affinity purified immunoglobulin
抗体製品タイプ
primary antibodies
クローン
polyclonal
精製方法
affinity chromatography
化学種の反応性
rat, mouse
メーカー／製品名
Chemicon®
テクニック
immunohistochemistry: suitable
western blot: suitable
NCBIアクセッション番号
UniProtアクセッション番号
輸送温度
wet ice
ターゲットの翻訳後修飾
unmodified
遺伝子情報
human ... SCN3A(6328)
特異性
Recognizes Nav1.3 (type III a subunit of VGSC, Scn3a). Does not cross react with any other sodium channel antigens tested so far.
免疫原
Purified peptide of alpha subunit (amino acids 511-524) of rat Nav1.3
( type III voltage-gated sodium channel VGSC)
( type III voltage-gated sodium channel VGSC)
アプリケーション
Research Category
ニューロサイエンス
ニューロサイエンス
Research Sub Category
イオンチャネル及びトランスポーター
イオンチャネル及びトランスポーター
All procedures that are going to receive a full-length protein should be performed at 4°C, and the following protease inhibitor mixture should be used: pepstatin A (1 μg/mL), leupeptin (1 μg/mL), aprotinin (1 μg/mL), Pefabloc SC (0.2 mM), benzamidine (0.1 mg/mL), and calpain inhibitors I and II (8 μg/mL each).
Western blot: 1:200 using ECL on newborn rat brain membranes.
Dilutions should be made using a carrier protein such as BSA (1-3%).
Immunohistochemistry: on fixed frozen sections.
Optimal working dilutions must be determined by the end user.
Western blot: 1:200 using ECL on newborn rat brain membranes.
Dilutions should be made using a carrier protein such as BSA (1-3%).
Immunohistochemistry: on fixed frozen sections.
Optimal working dilutions must be determined by the end user.
Detect Sodium Channel using this Anti-Sodium Channel Antibody, Voltage Gated, Brain Type III, pain validated for use in IH & WB.
物理的形状
Affinity purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA as a stabilizer and 0.05% sodium azide as a preservative. Reconstitute with 50 mL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min).
保管および安定性
Maintain lyophilized material at -20°C for up to 12 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
アナリシスノート
Control
CONTROL ANTIGEN: Included free of charge with the antibody is 40 μg of control antigen (lyophilized powder). The stock solution of the antigen can be made up using 100 μL of sterile deionized water. For negative control, preincubate 0.75 μg of peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
CONTROL ANTIGEN: Included free of charge with the antibody is 40 μg of control antigen (lyophilized powder). The stock solution of the antigen can be made up using 100 μL of sterile deionized water. For negative control, preincubate 0.75 μg of peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
その他情報
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法的情報
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
毒物及び劇物取締法
毒物
Jan Code
AB5208-200UL:
AB5208-50UL:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Frank H Yu et al.
Nature neuroscience, 9(9), 1142-1149 (2006-08-22)
Voltage-gated sodium channels (Na(V)) are critical for initiation of action potentials. Heterozygous loss-of-function mutations in Na(V)1.1 channels cause severe myoclonic epilepsy in infancy (SMEI). Homozygous null Scn1a-/- mice developed ataxia and died on postnatal day (P) 15 but could be
Sebastian K G Maier et al.
Circulation, 109(11), 1421-1427 (2004-03-10)
Voltage-gated sodium channels composed of pore-forming alpha and auxiliary beta subunits are responsible for the rising phase of the action potential in cardiac muscle, but their localizations have not yet been clearly defined. Immunocytochemical studies show that the principal cardiac
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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