おすすめの製品
由来生物
rabbit
品質水準
抗体製品の状態
affinity purified immunoglobulin
抗体製品タイプ
primary antibodies
クローン
polyclonal
精製方法
affinity chromatography
化学種の反応性
rat
メーカー／製品名
Chemicon®
テクニック
ELISA: suitable
immunohistochemistry: suitable
western blot: suitable
NCBIアクセッション番号
UniProtアクセッション番号
輸送温度
dry ice
ターゲットの翻訳後修飾
unmodified
遺伝子情報
human ... DRD1(1812)
特異性
Dopamine D1A Receptor (D1R). The rat D1A receptor is a 446 amino acid, G-protein coupled transmembrane receptor protein (Monsma, 1990; Zhou, 1990; Zhou, 1992; O′Dowd, 1990). The immunogen sequence has 88% sequence similarity with human and 77% with porcine.
免疫原
A nine amino acid peptide corresponding to the 4th extracellular domain of the rat D1A receptor. The immunogen is absolutely conserved in the mouse D1A receptor and shows 88% homology with human and 77% with porcine and bovine D1A receptors. The peptide shows no significant sequence similarity with other dopamine receptors (D1B, D2-D5).
Epitope: 4th Extracellular Domain
アプリケーション
Research Category
ニューロサイエンス
ニューロサイエンス
Research Sub Category
神経伝達物質及び受容体
神経伝達物質及び受容体
Detect Dopamine D1A Receptor using this Anti-Dopamine D1A Receptor Antibody, 4th Extracellular Domain validated for use in ELISA, IH & WB.
Immunohistochemistry: 2-10 μg/mL on paraformaldehyde fixed tissue sections. D1R has been localized in various regions of the rat brain (Huang, 1992; O′Connel, 1995)
Western blot: 1-10 μg/mL (Chemiluminescence technique). An antibody made to this peptide has been shown to detect 2 proteins of 50 and 75 kDa in solubilized hippocampal membranes. The 75 kDa band was not visible in striatum (Huang, 1992; O′Connel, 1995). Approximately 100-400 μg of protein/lane should be loaded to visualize the 75 kDa band (Huang, 1992; O′Connel, 1995) that is present in relatively small amounts.
ELISA: 0.5-1.0 μg/mL using 1 μg/mL of control peptide per well.
The immunogen peptide is available (Cat. No. AG266) for use in ELISA or antibody blocking studies. AG266 is not recommended for use as a western blot positive control.
Optimal working dilutions must be determined by end user.
Western blot: 1-10 μg/mL (Chemiluminescence technique). An antibody made to this peptide has been shown to detect 2 proteins of 50 and 75 kDa in solubilized hippocampal membranes. The 75 kDa band was not visible in striatum (Huang, 1992; O′Connel, 1995). Approximately 100-400 μg of protein/lane should be loaded to visualize the 75 kDa band (Huang, 1992; O′Connel, 1995) that is present in relatively small amounts.
ELISA: 0.5-1.0 μg/mL using 1 μg/mL of control peptide per well.
The immunogen peptide is available (Cat. No. AG266) for use in ELISA or antibody blocking studies. AG266 is not recommended for use as a western blot positive control.
Optimal working dilutions must be determined by end user.
物理的形状
Affinity purified immunoglobulin. Liquid in PBS with 0.2% BSA and 0.05% sodium azide.
保管および安定性
Maintain at -20°C in undiluted aliquots for up to 6 months from date of receipt. Avoid repeated freeze/thaw cycles.
アナリシスノート
Control
POSITIVE CONTROL: rat striatal membranes (western blot)
POSITIVE CONTROL: rat striatal membranes (western blot)
その他情報
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法的情報
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
10 - Combustible liquids
WGK
WGK 2
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
AB1784P:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Modification of dopaminergic markers expression in the striatum by neonatal exposure to glutamate during development.
S J Lopez-Perez, P Vergara, J P Ventura-Valenzuela, M E Ure?a-Guerrero, J Segovia, C Beas-Zarate
International Journal of Developmental Neuroscience null
Schizophrenia: dopamine D1 receptor sequence is normal, but has DNA polymorphisms.
Ohara, K, et al.
Neuropsychopharmacology, 8, 131-135 (1993)
Glenn S Belinsky et al.
Stem cells and development, 22(10), 1522-1540 (2013-01-05)
We tested whether dopaminergic drugs can improve the protocol for in vitro differentiation of H9 human embryonic stem cells (hESCs) into dopaminergic neurons. The expression of 5 dopamine (DA) receptor subtypes (mRNA and protein) was analyzed at each protocol stage
Estrogen protects against the synergistic toxicity by HIV proteins, methamphetamine and cocaine.
Turchan, J; Anderson, C; Hauser, KF; Sun, Q; Zhang, J; Liu, Y; Wise, PM; Kruman et al.
BMC Neuroscience null
Tyler W Stradleigh et al.
Progress in retinal and eye research, 48, 181-202 (2015-04-22)
Immunohistochemical and ex vivo anatomical studies have provided many glimpses of the variety, distribution, and signaling components of vertebrate retinal neurons. The beauty of numerous images published to date, and the qualitative and quantitative information they provide, indicate that these
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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