654245

TNF-α, Mouse, Recombinant, E. coli

• 別名: rmTNF-α, rm-Cachectin, Tumor Necrosis Factor-α
• UNSPSCコード: 12352202
• NACRES: NA.32

特性

• 由来生物: mouse
• 品質水準: 100
• リコンビナント: expressed in E. coli
• 詳細: Merck USA index - 14, 9812
• アッセイ: ≥97% (SDS-PAGE)
• フォーム: lyophilized
• 分子量: 17,000 g/mol
• メーカー／製品名: Calbiochem^®
• 保管条件: OK to freeze
• テクニック: cell based assay: suitable
• 不純物: ≤100 pg/μg Endotoxin (pg/μg TNF-α)
• 輸送温度: ambient
• 保管温度: −70°C
• 遺伝子情報: mouse ... Tnf(21926)

詳細

詳細

Reasearch area: Apoptosis

Recombinant mouse tumor necrosis factor-α (TNF-α), expressed in E. coli, is known for its direct tumoricidal capabilities. This inflammatory cytokine triggers a cascade of other cytokines and factors constituting the immune system′s response to infections and cancer. Additionally, it has been reported to influence changes in bone metabolism during inflammation. (TNF-α), a homotrimer protein, occurs in a soluble and transmembrane form.

アプリケーション

TNF-α, Mouse, Recombinant, E. coli has been used to study TNF-signaling to insulin gene expression in insulin-producing cells.

生物化学的／生理学的作用

TNF-α, a cytokine, interacts with two distinct receptors, initiating signal transduction pathways that result in cellular responses, such as cell survival, differentiation, and proliferation. It regulates inflammatory responses and activates inflammatory molecules, including other cytokines and chemokines. Transmembrane TNF-α binds to TNFR1 and TNFR2 and facilitates inflammation and cell death. However, excessive or inappropriate activation of TNF-α signaling is linked to chronic inflammation and may lead to pathological complications, including autoimmune diseases. Rheumatoid arthritis (RA), inflammatory bowel disease (IBD), psoriatic arthritis (PsA), psoriasis (PS), and noninfectious uveitis (NIU) are few conditions.

Additionally, TNF-α is associated with insulin signaling inhibition. Its removal ameliorates insulin sensitivity. The activity of the Jun N-terminal kinase (JNK) signaling pathway, which is abnormally elevated in obesity, is strongly activated by both TNF-α.

ED₅₀ = 20-50 pg/ml as measured in a cytotoxic assay using the TNF-α susceptible murine L-929 cell line in the presence of Actinomycin D (Cat. No. 114666)

警告

Toxicity: Standard Handling (A)

物理的形状

Lyophilized from sterile-filtered PBS, 50 µg BSA/µg TNF-α.

再構成

Following reconstitution, aliquot and freeze (-70°C) for long term storage or refrigerate (4°C) for short term storage. Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -70°C.

Reconstitute to a concentration ≥10 µg/ml with sterile PBS containing ≥0.1% HSA or BSA.

その他情報

Scharla, S.H., et al. 1994. Eur. J. Endocrinol. 131, 293.
Eck, M.J., et al. 1992. J. Biol. Chem. 267, 2119.

法的情報

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable