648310-M

トリス(ヒドロキシメチル)アミノメタン

≥99% (perchloric acid titration), solid, pH 7.0-9.0, solid, Molecular Biology Grade

• 別名: 2-アミノ-2-(ヒドロキシメチル)-1,3-プロパンジオール, THAM, トリス塩基, トロメタモール
• 化学式: NH₂C(CH₂OH)₃
• CAS番号: 77-86-1
• 分子量: 121.14
• Beilstein: 741883
• MDL番号: MFCD00004679
• UNSPSCコード: 12161705
• NACRES: NA.25

特性

• product name: トリス(ヒドロキシメチル)アミノメタン, TRIS base is useful in the pH range of 7.0-9.0. Has a pKa of 8.1 at 25°C.
• グレード: Molecular Biology
• 品質水準: 100
• アッセイ: ≥99% (perchloric acid titration)
• 形状: solid
• メーカー／製品名: Calbiochem^®
• 保管条件: OK to freeze
• 色: white
• 有効pH範囲: 7-9
• pKa (25 °C): 8.1
• bp: 219-220 °C/10 mmHg (lit.)
• mp: 167-172 °C (lit.)
• 溶解性: water: 500 mg/mL
• 微量陽イオン: heavy metals: ≤0.0005%
• 吸収: ≤0.02 at 280 nm; ≤0.03 at 260 nm
• その他の活性: RNases, DNases, proteases, none detected
• 保管温度: 10-30°C
• SMILES記法: NC(CO)(CO)CO
• InChI: 1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2
• InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N

詳細

詳細

Useful in the pH range of 7.0-9.0. Has a pK_a of 8.1 at 25°C.

Widely used as a biological buffer (absorbance: ≤0.03 at 260 nm; ≤0.02 at 280 nm). Useful in the pH range of 7.0-9.0. Has a pK_a of 8.1 at 25°C.

アプリケーション

• A Fast and Automated Melanin-bleaching Method for Histopathologic Evaluation of Pigmented Melanoma Tissues.: This study presents an automated melanin-bleaching technique using a Tris-based buffer to enhance histopathologic evaluation of pigmented melanoma tissues, aiding in more accurate diagnoses (Ugolini et al., 2022).


• Improvement of plant protein solubilization and 2-DE gel resolution through optimization of the concentration of Tris in the solubilization buffer.: Highlights the optimization of Tris concentration in solubilization buffers to enhance protein solubilization and gel electrophoresis resolution, crucial for biochemical studies (Cho et al., 2010).

警告

Toxicity: Irritant (B)

法的情報

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable