567704

Sphingolipid Ceramide N-Deacylase, Pseudomonas sp.

• 別名: SCDase
• UNSPSCコード: 12352202
• NACRES: NA.77

特性

• 由来生物: bacterial (Pseudomonas spp.)
• 品質水準: 100
• フォーム: liquid
• 比活性: ≥1 units/mL
• メーカー／製品名: Calbiochem^®
• 保管条件: OK to freeze; avoid repeated freeze/thaw cycles
• その他の活性: α- and β-N-acetylgalactosaminidase, α-fucosidase, α- and β-galactosidase, neuraminidases, none detected
• 輸送温度: wet ice
• 保管温度: −20°C

詳細

詳細

Sphingolipid ceramide N-deacylase (SCDase) is a native sphingolipid ceramide N-deacylase from Pseudomonas species. It hydrolyzes the N-acyl linkage between fatty acids and the sphingosine base of ceramide in several sphingolipids and gangliosides. This enzyme catalyzes the condensation reaction between fatty acids and sphingosine bases and forms sphingolipids. It is also involved in transacylation reactions. SCDase is not recommended for hydrolysis of ceramide. It is also used to prepare lysoglycosphingolipids (lysoGSLs) effortlessly.

アプリケーション

Sphingolipid ceramide N-Deacylase, Pseudomonas sp. has been used as a PP2A enhancer to study its effects on phosphatase and tensin homolog (PTEN) in forkhead box O3 (FOXO3a) phosphorylation in primary CD4⁺ T cells.

警告

Toxicity: Standard Handling (A)

単位の定義

One unit is defined as the amount of enzyme that will hydrolyze 1 µmol of asialo-GM₁ per min at 37°C, pH 6.0.

物理的形状

In 50 mM sodium acetate buffer, 0.1% LUBROL PX Detergent, pH 6.0.

再構成

Following initial thaw, aliquot and freeze (-20°C).

その他情報

Mitsutake, S., et al. 1997. Anal. Biochem. 247, 52.
Sueyoshi, N., et al. 1997. J. Lipid Res. 38, 1923.
Ito, M., et al. 1995. J. Biol. Chem. 270, 24370.

法的情報

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable