480010-M

NDSB-256

A non-detergent sulfobetaine. Reported to prevent protein aggregation and facilitate the renaturation of chemically and thermally denatured proteins.

• 別名: NDSB-256, Dimethylbenzylammonium Propane Sulfonate
• 実験式（ヒル表記法）: C₁₂H₁₉NO₃S
• CAS番号: 81239-45-4
• 分子量: 257.35
• MDL番号: MFCD00225018
• UNSPSCコード: 12161902
• NACRES: NA.21

特性

• 品質水準: 100
• アッセイ: ≥99% (TLC)
• 形状: solid
• メーカー／製品名: Calbiochem^®
• 保管条件: OK to freeze; desiccated (hygroscopic)
• 色: white
• 溶解性: water: 200 mg/mL
• 保管温度: 10-30°C
• InChI: 1S/C12H19NO3S/c1-13(2,9-6-10-17(14,15)16)11-12-7-4-3-5-8-12/h3-5,7-8H,6,9-11H2,1-2H3
• InChI Key: MEJASPJNLSQOAG-UHFFFAOYSA-N

詳細

詳細

A member of the class of nondetergent sulfobetaines. Unlike CHAPS and other zwittergents, NDSBs are nondenaturing even at 1 M concentrations. NDSBs have been used to improve the efficiency of solubilization of membrane proteins, to isolate nuclear proteins, to chromatographically purify halophilic proteins in low ionic strength buffers, and to crystallize proteins. They are zwitterionic over a wide pH range and do not absorb significantly in the near UV range. Reported to prevent protein aggregation and facilitate the renaturation of chemically and thermally denatured proteins. NDSBs can be easily removed by dialysis. At 1 M, NDSB-256 will restore 30% of the enzymatic activity of denatured egg white lysozyme; at 800 mM it will restore 16% of the enzymatic activity of denatured β-galactosidase.

A non-detergent sulfobetaine. Reported to prevent protein aggregation and facilitate the renaturation of chemically and thermally denatured proteins. Zwitterionic over a wide pH range. Easily removed by dialysis. At 1 M, NDSB-256 will restore 30% of the enzymatic activity of denatured egg white lysozyme; at 800 mM it will restore 16% of the enzymatic activity of denatured β-galactosidase.

警告

Toxicity: Irritant (B)

再構成

Following reconstitution, store at room temperature (15-30°C). Stock solutions are stable for up to 3 months at room temperature.

その他情報

Goldberg, M.E., et al. 1996. Folding & Design1, 21.

法的情報

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable