220201

CHAPS

Molecular Biology Grade

• 別名: 3-[(3-コールアミドプロピル)ジメチルアンモニオ]-1-プロパンスルホン酸
• 実験式（ヒル表記法）: C₃₂H₅₈N₂O₇S
• CAS番号: 75621-03-3
• 分子量: 614.88
• MDL番号: MFCD00012116
• UNSPSCコード: 12161703
• NACRES: NA.25

特性

• product name: CHAPS,
• グレード: Molecular Biology
• 品質水準: 300
• 詳細: zwitterionic
• アッセイ: ≥98% (NMR)
• 形状: crystalline powder
• 分子量: micellar avg mol wt 6150
• メーカー／製品名: Calbiochem^®
• 保管条件: OK to freeze; desiccated (hygroscopic)
• 凝集数: 10
• 色: white
• CMC: 6 - 10 mM; 6 mM (20-25°C)
• mp: 157 °C (315 °F)
• 転移温度: cloud point >100 °C
• 溶解性: water: 1.0 M
• その他の活性: DNase, RNase, and Protease, none detected
• 輸送温度: ambient
• 保管温度: 15-25°C
• SMILES記法: C[C@H](CCC(=O)NCCC[N+](C)(C)CCCS([O-])(=O)=O)[C@H]1CC[C@H]2[C@@H]3[C@H](O)CC4C[C@H](O)CC[C@]4(C)[C@H]3C[C@H](O)[C@]12C
• InChI: 1S/C32H58N2O7S/c1-21(8-11-29(38)33-14-6-15-34(4,5)16-7-17-42(39,40)41)24-9-10-25-30-26(20-28(37)32(24,25)3)31(2)13-12-23(35)18-22(31)19-27(30)36/h21-28,30,35-37H,6-20H2,1-5H3,(H-,33,38,39,40,41)/t21-,22?,23-,24-,25+,26+,27-,28+,30+,31+,32-/m1/s1
• InChI Key: UMCMPZBLKLEWAF-RFCNGIAKSA-N

詳細

詳細

Conductivity (1.0 M, 24°C): <100 µmhos.

Non-denaturing zwitterionic detergent that combines features of bile salts and N-alkyl sulfobetaines. Better at solubilizing proteins than the structurally related carboxylic acid anions, and is more effective at breaking protein-protein interactions than either sodium cholate or TRITON^™ X-100. Capable of solubilizing the opiate receptor to a state exhibiting reversible binding to opiates. Also capable of disaggregating P 450 to its monomeric form without denaturation. Has a small micellar molecular weight (6150 Daltons) and a high critical micellar concentration (6-10 mM). Can be removed from either gels or protein solutions by dialysis. Aggregation number: 4-14.

Zwitterionic detergent that combines features of bile salts and N-alkyl sulfobetaines. Better at solubilizing proteins than structurally-related carboxylic acid anions and is much more effective at breaking protein-protein interactions than either sodium cholate or TRITON^™ X-100 Detergent. Capable of solubilizing the opiate receptor to a state exhibiting reversible binding of opiates. Also, capable of disaggregating cytochrome P450 to its monomeric form without denaturation. Has a small micellar molecular weight and a high critical micellar concentration and can be removed from either gels or protein solutions by dialysis across a membrane. Aggregation number: 4-14.

アプリケーション

CHAPSは非変性性の双性イオン界面活性剤で、膜の生化学研究に用いられます。
膜タンパク質の可溶化やタンパク質-タンパク質相互作用の阻害に有用です。CHAPSはミセルの分子量が小さく(6,150)、限界ミセル濃度が高い(6~10 mM)ため、透析によってサンプルから除去することが可能です。等電点電気泳動(IEF)および二次元電気泳動の際のタンパク質可溶化にも適しています。CHAPSは非変性の(尿素を使用しない)IEFで一般に用いられており、細胞内画分や植物タンパク質の分離で優れた性能を示しています。IEFゲルにおける通常の使用濃度は2~4% (w/v)です。

警告

Toxicity: Harmful (C)

再構成

Following reconstitution, store in the refrigerator (4°C). Stock solutions are stable for up to 6 months at 4°C.

その他情報

Chen, Y., et al. 1992. Biochemistry 31, 2415.
Ofri, D., et al. 1992. J. Neurochem. 58, 628.
Ransom, R.W., et al. 1992. Biochem. Pharmacol. 43, 1823.
Simonds, W.F., et al. 1992. Proc. Natl. Acad. Sci. USA77, 4623.
Yannariello-Brown, J., and Weigel, P.H. 1992. Biochemistry31, 576.
Hjelmeland, L.M. 1980. Proc. Natl. Acad. Sci. USA77, 6368.
Simonds, W.F., et al. 1980. Proc. Natl. Acad. Sci. USA77, 4623.

法的情報

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

安全性情報

• ピクトグラム: GHS07
• シグナルワード: Warning
• 危険有害性情報: H302
• 注意書き: P264 - P270 - P301 + P312 - P501
• 危険有害性の分類: Acute Tox. 4 Oral
• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable