444605

Melittin

A 26-residue polypeptide from bee venom that binds calmodulin in a Ca²⁺-dependent manner.

• 別名: Melittin
• 実験式（ヒル表記法）: C₁₃₁H₂₂₉N₃₉O₃₁
• CAS番号: 37231-28-0
• 分子量: 2846.46
• UNSPSCコード: 12352200
• NACRES: NA.77

特性

• 品質水準: 100
• アッセイ: >97% (HPLC)
• 形状: lyophilized
• メーカー／製品名: Calbiochem^®
• 保管条件: OK to freeze
• 溶解性: water: 1 mg/mL
• 輸送温度: ambient
• 保管温度: −20°C
• InChI: 1S/C131H229N39O31/c1-23-71(16)102(163-97(176)60-135)122(194)146-62-98(177)148-74(19)109(181)164-100(69(12)13)124(196)160-88(55-65(4)5)116(188)155-84(41-30-33-51-134)115(187)165-101(70(14)15)125(197)161-90(57-67(8)9)118(190)168-106(77(22)173)128(200)169-105(76(21)172)123(195)147-63-99(178)150-92(58-68(10)11)129(201)170-54-36-44-94(170)121(193)149-75(20)108(180)158-89(56-66(6)7)117(189)166-104(73(18)25-3)127(199)162-93(64-171)120(192)159-91(59-78-61-145-80-38-27-26-37-79(78)80)119(191)167-103(72(17)24-2)126(198)157-83(40-29-32-50-133)111(183)154-85(42-34-52-143-130(139)140)112(184)152-82(39-28-31-49-132)110(182)153-86(43-35-53-144-131(141)142)113(185)156-87(46-48-96(137)175)114(186)151-81(107(138)179)45-47-95(136)174/h26-27,37-38,61,65-77,81-94,100-106,145,171-173H,23-25,28-36,39-60,62-64,132-135H2,1-22H3,(H2,136,174)(H2,137,175)(H2,138,179)(H,146,194)(H,147,195)(H,148,177)(H,149,193)(H,150,178)(H,151,186)(H,152,184)(H,153,182)(H,154,183)(H,155,188)(H,156,185)(H,157,198)(H,158,180)(H,159,192)(H,160,196)(H,161,197)(H,162,199)(H,163,176)(H,164,181)(H,165,187)(H,166,189)(H,167,191)(H,168,190)(H,169,200)(H4,139,140,143)(H4,141,142,144)
• InChI Key: VDXZNPDIRNWWCW-UHFFFAOYSA-N

詳細

詳細

A 26-residue polypeptide from bee venom that binds calmodulin in a Ca²⁺-dependent manner, activates phospholipase A₂, and inhibits protein kinase C (IC₅₀ = 5-7 µM) by binding to the catalytic domain in a Mg²⁺-ATP sensitive manner. A stimulator of G_iα and G₁₁α activity that is reported to inhibit adenylate cyclase activity in synaptic membranes. Has also been used for affinity purification of several Ca²⁺-binding proteins. Activates G_iα-1 and G₁₁α activities and inhibits G_s activity.

A 26-residue polypeptide from bee venom that binds calmodulin in a Ca²⁺-dependent manner. A stimulator of G_iα and G₁₁α activity that is reported to inhibit adenylate cyclase activity in synaptic membranes. Activates phospholipase A₂ and inhibits protein kinase C (IC₅₀ = 5-7 µM) by binding to the catalytic domain in a Mg²⁺-ATP sensitive manner. Has also been used for affinity purification of several Ca²⁺-binding proteins.

生物化学的／生理学的作用

Cell permeable: no

Product does not compete with ATP.

Reversible: no

Target IC₅₀: 5-7 µM 5-7 µM for activating phospholipase A2 and inhibits protein kinase C

包装

Yes

警告

Toxicity: Standard Handling (A)

シーケンス

H-Gly-Ile-Gly-Ala-Val-Leu-Lys-Val-Leu-Thr-Thr-Gly-Leu-Pro-Ala-Leu-Ile-Ser-Trp-Ile-Lys-Arg-Lys-Arg-Gln-Gln-NH₂

その他情報

Fukushima, N., et al. 1998. Peptides19, 811.
Gravitt, K.R. 1994. Biochem. Pharmacol. 47, 425.
Baudier, J., et al. 1987. Biochemistry26, 2886.
Kincaid, R.L., and Coulson, C.C. 1985. Biochem. Biophys. Res. Commun.133, 256.

法的情報

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 3