219467

Cathepsin V, His•Tag^®, Human, Recombinant, NSO Cells

recombinant human, expressed in NSO cells

• 別名: Cathepsin L2, Cathepsin U
• Enzyme Commission number: 3.4.22.43 (BRENDA, IUBMB)
• UNSPSCコード: 12352202
• NACRES: NA.77

特性

• リコンビナント: expressed in NSO cells
• 品質水準: 100
• アッセイ: ≥95% (SDS-PAGE)
• 形状: frozen liquid
• 比活性: ≥300 units/μg
• メーカー／製品名: Calbiochem^®
• 保管条件: OK to freeze; avoid repeated freeze/thaw cycles
• 不純物: ≤1.0 EU/μg Endotoxins (EU/μg Cathepsin V)
• 輸送温度: wet ice
• 保管温度: −70°C

詳細

詳細

Recombinant, human procathepsin V (amino acids 18-334) fused at the N-terminus to a CD33 signal sequence (amino acids 1-16) and at the C-terminus to a His•Tag sequence and expressed in NSO cells. Cat V is predominantly expressed in normal thymus, testis, and corneal epithelium. It is involved in the invariant chain degradation in thymic epithelial cells and its overexpression has been detected in myasthenia gravis. Abnormally wide spread expressions in mammary gland and colon have also been reported in breast and colonrectal carcinomas. Although it shows high amino acid identity with cathepsin L (Cat. No. 219382), these two cathepsins have very different tissue distribution and substrate specificity. As a result of glycosylation, this recombinant enzyme migrates ~40 kDa on SDS-PAGE under either reducing or non-reducing conditions. Requires activation prior to use.

Recombinant, human procathepsin V (amino acids 18-334) fused at the N-terminus to a CD33 signal sequence (amino acids 1-16) and at the C-terminus to a His•Tag sequence and expressed in NSO cells. Cat V is predominantly expressed in normal thymus, testis, and corneal epithelium. It is involved in the invariant chain degradation in thymic epithelial cells and its overexpression has been detected in myasthenia gravis. Abnormally wide spread expressions in mammary gland and colon have also been reported in breast and colonrectal carcinomas. Although it shows high amino acid identity with cathepsin L (Cat. No. 219382), these two cathepsins have very different tissue distribution and substrate specificity. As a result of glycosylation, this recombinant enzyme migrates ~40 kDa on SDS-PAGE under either reducing or non-reducing conditions. Requires activation prior to use.

包装

Please refer to vial label for lot-specific concentration.

警告

Toxicity: Standard Handling (A)

単位の定義

One unit is defined as the amount of enzyme that will cleave 1 pmol the fluorescent substrate Z-LR-AMC per min at 25°C, pH 5.5.

物理的形状

In 800 mM NaCl, 25 mM sodium acetate, 0.8 M NaCl, pH 5.0.

再構成

To activate rhCathepsin V, pre-incubate it at 20 µg/ml in 25 mM NaOAc, 100 mM NaCl, 5 mM DTT, pH 5.5 at room temperature. Following initial thaw aliquot and freeze (-70°C).

その他情報

Tolosa, E., et al. 2003. J. Clin. Invest.112, 517.
Turk, V., et al. 2001. EMBO J.20, 4629.
Somoza, J.R., et al. 2000. Biochemistry39, 12543.
Bromme, D., et al. 1999. Biochemistry38, 2377.
Adachi, W., et al. 1998. Invest. Ophthalmol. Vis. Sci.39, 1789.
Santamaria, I., et al. 1998. Cancer Res.58, 1624.

法的情報

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable