208773

Calpain Substrate IV, Cell-Permeable, Fluorogenic

The non-permeant fluorogenic Calpain Substrate III is made cell-permeable by a c-terminal heptaarginine extension via a Glu linkage.

• 別名: Calpain Substrate IV, Cell-Permeable, Fluorogenic
• 実験式（ヒル表記法）: C₁₂₇H₂₀₆N₅₀O₂₇S
• 分子量: 2897.38
• UNSPSCコード: 12352200
• NACRES: NA.77

特性

• 品質水準: 100
• アッセイ: ≥95% (HPLC)
• 形状: lyophilized solid
• メーカー／製品名: Calbiochem^®
• 保管条件: OK to freeze; desiccated (hygroscopic); protect from light
• 溶解性: DMSO: 5 mg/mL
• 蛍光検出: λ_ex 480 nm; λ_em 320 nm
• 輸送温度: ambient
• 保管温度: −20°C

詳細

詳細

The non-permeant fluorogenic Calpain Substrate III (Cat. No. 208771) is made cell-permeable by a c-terminal heptaarginine extension via a Glu linkage. In addition to the expected improvement in permeability, the modified peptide also exhibits enhanced Calpain B sensitivity (k_cat = 0.2 s^-1, k_cat/K_M = 5000 M^-1 s^-1) when compared to the parental peptide (k_cat = 0.17 s^-1, k_cat/K_M = 680 M^-1 s^-1). Its suitability in detecting cellular Ca²⁺-dependent calpain activity is demonstrated both in intact Drosophila melanogaster S2 cells following ionomycin stimulation and in S2 lysates via direct Ca²⁺ addition.

The non-permeant fluorogenic Calpain Substrate III (Cat. No. 208771) is made cell-permeable by a c-terminal heptaarginine extension via a Glu linkage. In addition to the expected improvement in permeability, the modified peptide also exhibits enhanced Calpain B sensitivity (k_cat = 0.2 s^-1, k_cat/K_M = 5000 M^-1 s^-1) when compared to the parental peptide (k_cat = 0.17 s^-1, k_cat/K_M = 680 M^-1 s^-1). Its suitability in detecting cellular Ca²⁺-dependent calpain activity is demonstrated both in intact Drosophila melanogaster S2 cells following ionomycin stimulation and in S2 lysates via direct Ca²⁺ addition.

The non-permeant fluorogenic Calpain Substrate III is made cell-permeable by a c-terminal heptaarginine extension via a Glu linkage.

包装

Packaged under inert gas

警告

Toxicity: Standard Handling (A)

シーケンス

(DABCYL)-Thr-Pro-Leu-Lys~Ser-Pro-Pro-Pro-Ser-Pro-Arg-Glu(EDANS)-Arg-Arg-Arg-Arg-Arg-Arg-Arg-NH₂

物理的形状

Supplied as a trifluoroacetate salt

再構成

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

その他情報

Banoczi, Z., et al. 2008. Bioconjug. Chem.19, 1375.

法的情報

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable