09-481

Anti-PI3 Kinase Antibody, p110α

from rabbit, purified by affinity chromatography

• 別名: PI3-kinase p110 subunit alpha, PtdIns-3-kinase p110, phosphatidylinositol 3-kinase, catalytic, 110-KD, alpha, phosphatidylinositol 3-kinase, catalytic, alpha polypeptide, phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit, alpha isoform, ph
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: rabbit
• 品質水準: 100
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 精製方法: affinity chromatography
• 化学種の反応性: human, rat
• テクニック: ELISA: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• NCBIアクセッション番号: NP_006209.2
• UniProtアクセッション番号: P42336
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... PIK3CA(5290) ; rat ... Pik3Ca(170911)

詳細

詳細

Phosphoinositol-3-kinases (PI3 Kinase, PI3K) are a family of lipid kinases that mediate many intracellular signaling responses in both physiological and pathophysiological states. PI3 Kinase is a heteroduplex with three classes (I, II, & III) of catalytic domain. The class I PI3 Kinases are made up of a p85 regulatory protein and a p110 catalytic domain. There are 4 isoforms of the class I p110 catalytic domains. Class I(A) include α, β, γ and class I(B). They are activated downstream via receptor tyrosine kinases or G-protein coupled receptors. Once activated, PI3K generates phosphatidylinositol 3,4,5-trisphosphate (PIP3) from PIP2. It was later found that PTEN, a lipid phosphatase, acts opposite to PI3K by dephosphorylating PIP3, converting it to PIP2, thus de-activating the effects of PI3K. Increased levels of PIP3 in the cell leads to the activation of many key signaling regulators including Akt and PDK as well as calcium release. One of the primary roles of PI3K is its regulation of cell growth and cell metabolism. Both of these play a critical role in cancer as some believe that a tumor’s shift to glycolysis is due to the activation of PI3K. PI3 kinases have been linked with numerous disease states, including cancer, diabetes, allergic response, hypertension, cardiac contractility, atherosclerosis, sepsis and autoimmune/ inflammatory disorders.

特異性

Recognizes PI3 Kinase p110α.

免疫原

Epitope: Internal

KLH-conjugated synthetic linear peptide corresponding to an internal region of class I catalytic domain of PI3 Kinase that is specific to the p110 alpha isoform.

アプリケーション

Research Category
細胞シグナル伝達

Research Sub Category
PI3K、Akt及びmTORシグナル伝達

Detect PI3 Kinase using this Anti-PI3 Kinase Antibody, p110α validated for use in WB, IP, IH, ELISA.

品質

Evaluated by western blot on Jurkat cell extract.

Western Blotting Analysis: 0.5 µg/mL (1:2,000) dilution of this antibody detected PI3K, p110α in Jurkat cell extract.

ターゲットの説明

110 kDa

関連事項

Replaces: 04-399

物理的形状

Immunogen Affinity Purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl, and 0.05% sodium azide.

調製ノート

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl, and 0.05% sodium azide.

保管および安定性

Stable for 1 year at 2-8ºC from date of receipt.

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable