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Key Documents

安全性情報

05-212

Sigma-Aldrich

Anti-PI3 Kinase Antibody, p85, N-SH3, clone AB6

clone AB6, Upstate®, from mouse

別名:

PI3-kinase p85 subunit alpha, PtdIns-3-kinase p85-alpha, phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 1 (p85

alpha), phosphatidylinositol 3-kinase, regulatory, 1, phosphatidylinositol 3-kinase-associated p-85 alpha, phosphoinos

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About This Item

UNSPSCコード:
12352203
eCl@ss:
32160702
NACRES:
NA.41

由来生物

mouse

品質水準

抗体製品の状態

purified antibody

抗体製品タイプ

primary antibodies

クローン

AB6, monoclonal

化学種の反応性

human, rat, mouse

メーカー/製品名

Upstate®

テクニック

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

アイソタイプ

IgG1κ

NCBIアクセッション番号

UniProtアクセッション番号

輸送温度

wet ice

ターゲットの翻訳後修飾

unmodified

遺伝子情報

human ... PIK3R1(5295)

詳細

The enzyme phosphatidylinositol 3 kinase (PI3 kinase) is a lipid kinase that is thought to phosphorylate second messengers in growth signaling pathways. PI3K exists as a heterodimer of catalytic 110 kDa (p110) and regulatory 85 kDa (p85, p50 or p55) subunits. The p85 unit consists of two closely related proteins: p85 alpha, which binds tightly to the catalytic subunit and p85 beta, a protein of unknown function. They share 62% amino acid sequence identity. p85 alpha lacks PI3 kinase activity, but may serve as regulator of the catalytic subunit, p110, by acting as the link between PI3K and the ligand activated receptor. Both isoforms bind to activated receptors and serve as tyrosine kinase substrates.

特異性

This antibody recognizes p85 alpha subunit of PI3 Kinase; does not recognize p85 beta subunit.

免疫原

Recombinant human p85 alpha PI3 Kinase expressed in E. Coli. Clone AB6.

アプリケーション

Research Category
細胞シグナル伝達
Research Sub Category
PI3K、Akt及びmTORシグナル伝達

キナーゼ及びホスファターゼ
Immunocytochemistry:
10 µg/mL of a previous lot of this antibody was used in immunocytochemistry.

Immunoprecipitation:
4 µg of a previous lot immunoprecipitated PI3 Kinase from 500 µg of an A431 RIPA lysate, as confirmed by immunoblot using a polyclonal PI3 Kinase antibody (Catalog # 06-195).
This Anti-PI3 Kinase Antibody, p85, N-SH3, clone AB6 is validated for use in IC, IP, WB for the detection of PI3 Kinase.

品質

Routinely evaluated by Western Blot on RIPA lysates from human A431 cells, or RIPA lysates from rat L6 myoblasts.

Western Blot Analysis:
0.5-2 µg/mL of this lot detected PI3 Kinase in RIPA lysates from human A431 cells; previous lots detected PI3 Kinase in RIPA lysates from rat L6 myoblasts.

ターゲットの説明

85 kDa

関連事項

Replaces: 04-403

物理的形状

Protein A chromatography
Format: Purified
Purified mouse monoclonal IgG1κ in buffer containing PBS, pH 7.2 with 50% glycerol.

保管および安定性

Stable for 1 year at -20°C from date of receipt.

Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20ºC. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20ºC may cause glycerol-containing solutions to become frozen during storage.

アナリシスノート

Control
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法的情報

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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保管分類コード

12 - Non Combustible Liquids

WGK

WGK 2

引火点(°F)

Not applicable

引火点(℃)

Not applicable


適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

Jan Code

05-212:


試験成績書(COA)

製品のロット番号・バッチ番号を入力して、試験成績書(COA) を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。

以前この製品を購入いただいたことがある場合

文書ライブラリで、最近購入した製品の文書を検索できます。

文書ライブラリにアクセスする

Erythropoietin-dependent association of phosphatidylinositol 3-kinase with tyrosine-phosphorylated erythropoietin receptor.
Miura, O, et al.
The Journal of Biological Chemistry, 269, 614-620 (1994)
Structure of 85 kDa subunit of human phosphatidylinositol 3-kinase analyzed by using monoclonal antibodies
Tanaka, S., et al
Japanese Journal of Cancer Research, 84, 279-289 (1993)
Kohjiro Ueki et al.
Molecular and cellular biology, 22(3), 965-977 (2002-01-11)
Class Ia phosphoinositide (PI) 3-kinase is a central component in growth factor signaling and is comprised of a p110 catalytic subunit and a regulatory subunit, the most common family of which is derived from the p85alpha gene (Pik3r1). Optimal signaling
Differential activation of phospholipase C-gamma 1 and mitogen-activated protein kinase in naive and antigen-primed CD4 T cells by the peptide/MHC ligand
Ericsson, P. O., et al
Journal of immunology (Baltimore, Md. : 1950), 156, 2045-2053 (1996)
Cyril Basquin et al.
Journal of cell science, 126(Pt 5), 1099-1108 (2013-01-25)
Receptor-mediated endocytosis is an essential process used by eukaryotic cells to internalise many molecules. Several clathrin-independent endocytic routes exist, but the molecular mechanism of each pathway remains to be uncovered. The present study focuses on a clathrin-independent dynamin-dependent pathway used

ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.

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