05-746R
Anti-phospho-Histone H3 (Thr3) Antibody, clone JY325, rabbit monoclonal
clone JY325, from rabbit
別名:
Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/I, Histone H3/j, Histone H3/k, Histone H3/l
ログイン組織・契約価格を表示する
すべての画像(1)
About This Item
UNSPSCコード:
12352203
eCl@ss:
32160702
NACRES:
NA.41
おすすめの製品
由来生物
rabbit
品質水準
抗体製品の状態
purified immunoglobulin
抗体製品タイプ
primary antibodies
クローン
JY325, monoclonal
化学種の反応性
human
包装
antibody small pack of 25 μL
テクニック
inhibition assay: suitable (peptide)
western blot: suitable
アイソタイプ
IgG
NCBIアクセッション番号
UniProtアクセッション番号
輸送温度
dry ice
ターゲットの翻訳後修飾
phosphorylation (pThr3)
遺伝子情報
human ... HIST1H3F(8968)
詳細
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. High levels of phosphorylation of Histone H3 are associated with mitosis. Consistent with this role, Histone H3 phosphorylated at threonine 3 has been shown to be recognized by the BIR domain Survivin, a member of the inhibitor of apoptosis (IAP) family.
特異性
This antibody recognizes Histone H3 phosphorylated at Thr3.
免疫原
Epitope: Phosphorylated Thr3
KLH-conjugated linear peptide corresponding to human Histone H3 phosphorylated at Thr3.
アプリケーション
Research Category
エピジェネティクス及び核内機能分子
エピジェネティクス及び核内機能分子
Research Sub Category
ヒストン
ヒストン
Anti-phospho-Histone H3 (Thr3) Antibody, clone JY325 is a Rabbit Monoclonal Antibody for detection of phospho-Histone H3 (Thr3) also known as Histone H3.1 & has been validated in WB, PIA.
Peptide Block Analysis: This antibody peptide blocked on colcemid treated HeLa cell extracts.
品質
Evaluated by Western Blot using colcemid treated HeLa acid extract and recombinant Histone H3 negative control.
Western Blot Analysis: 1:8,000 dilution of this antibody detected Histone H3 in 10 µg of colcemid treated HeLa acid extract.
Western Blot Analysis: 1:8,000 dilution of this antibody detected Histone H3 in 10 µg of colcemid treated HeLa acid extract.
ターゲットの説明
~17 kDa observed
物理的形状
Protein A
Format: Purified
Purified rabbit monoclonal IgG in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide and 40% glycerol.
保管および安定性
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
アナリシスノート
Control
Colcemid treated HeLa acid extract and recombinant Histone H3 negative control.
Colcemid treated HeLa acid extract and recombinant Histone H3 negative control.
その他情報
Please refer to lot specific datasheet.
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
適切な製品が見つかりませんか。
製品選択ツール.をお試しください
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Laurence de Nijs et al.
Human molecular genetics, 21(23), 5106-5117 (2012-08-29)
Heterozygous mutations in Myoclonin1/EFHC1 cause juvenile myoclonic epilepsy (JME), the most common form of genetic generalized epilepsies, while homozygous F229L mutation is associated with primary intractable epilepsy in infancy. Heterozygous mutations in adolescent JME patients produce subtle malformations of cortical
Lama Tarayrah et al.
Biology open, 4(11), 1518-1527 (2015-10-23)
Signaling pathways and epigenetic mechanisms have both been shown to play essential roles in regulating stem cell activity. While the role of either mechanism in this regulation is well established in multiple stem cell lineages, how the two mechanisms interact
Analysis of Histones H3 and H4 Reveals Novel and Conserved Post-Translational Modifications in Sugarcane.
Moraes, I; Yuan, ZF; Liu, S; Souza, GM; Garcia, BA; Casas-Mollano, JA
Testing null
Brendan Evano et al.
Cell reports, 30(10), 3195-3206 (2020-03-12)
Stem cells can be maintained through symmetric cell divisions (SCDs) and asymmetric cell divisions (ACDs). How and when these divisions occur in vivo in vertebrates is poorly understood. Here, we developed a clonogenic cell tracing method that demonstrates the asymmetric distribution
Toshihiro Inubushi et al.
PLoS genetics, 18(7), e1009765-e1009765 (2022-07-16)
Hyaluronan (HA) is a major extracellular matrix component whose tissue levels are dynamically regulated during embryonic development. Although the synthesis of HA has been shown to exert a substantial influence on embryonic morphogenesis, the functional importance of the catabolic aspect
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
製品に関するお問い合わせはこちら（テクニカルサービス）