T511552

PTAD-Azide

Aldrich^CPR

• 別名: 4-(4-(2-Azidoethoxy)phenyl)-1,2,4-triazolidine-3,5-dione, N_3-Ph-Ur for e-Y-CLICK
• 実験式（ヒル表記法）: C₁₀H₁₀N₆O₃
• CAS番号: 1313211-52-7
• 分子量: 262.22
• MDL番号: MFCD25976544
• UNSPSCコード: 12352125
• PubChem Substance ID: 329826813

特性

• SMILES記法: O=C(NNC1=O)N1C2=CC=C(OCCN=[N+]=[N-])C=C2
• InChI: 1S/C10H10N6O3/c11-15-12-5-6-19-8-3-1-7(2-4-8)16-9(17)13-14-10(16)18/h1-4H,5-6H2,(H,13,17)(H,14,18)
• InChI Key: MHGMHPVYCVQIET-UHFFFAOYSA-N

詳細

アプリケーション

PTAD-Azide is a selective bioconjugation reagent for the modification of tyrosine residues with an azide. Subsequent modification can then be afforded via the azide/alkyne click chemistry reaction with an alkyne or cyclooctyne containing compound or biomolecule. This reagent has been shown to selectively introduce poly(ethylene glycol) or PEG chains onto proteins with surface exposed tyrosine residues. PTAD-Azide has also been used in the formation of antibody-drug conjugates. This reagent is compatible with different buffer systems such as PBS, Tris and mixed PBS/Tris buffer (preferred). The linkage with tyrosine has been shown to be stable to pH and temperature extremes as well as blood plasma.
   
Note: PTAD-Azide must be first activated by stirring in a 1:0.98 molar ratio with 1,3-dibromo-5,5-dimethylhydantoin (product # 157902). Activation is evident upon solution color change from colorless to deep red and the activated reagent should be used immediately.
 
 
General Procedure for Protein Modification with PTAD.
 
Part 1: PTAD activation

• Mix together 1:0.98 molar equivalents of unactivated PTAD to 1,3-dibromo-5,5-dimethylhydantoin (product # 157902) in organic solvent (preferred solvents are DMF or acetonitrile; avoid using DMSO)
• Color change is observed from colorless/pale yellow to deep red (approximately 5 min of mixing).
• After the solution turns red, store the now activated reagent on ice and use for protein modification within 30 min.

 
Part 2: Protein modification

• Add protein solution in mixed phosphate/Tris buffer or Tris buffer (pH should be 6 - 9) to the eppendorf tube (or other vial) containing the activated PTAD reagent prepared above and mix gently at room temperature for up to 30 min. Preferably use 10-fold molar excess of reagent relative to protein. Use protein at a minimum concentration of 1 mg/ml (higher concentrations are preferred for enhanced labeling).
• Remove excess unreacted PTAD by gel filtration.

包装

Each AldrichCPR product will be packaged in one consolidated unit unless you specify multiple units at the time of order.

その他情報

Sigma-Aldrichは、ユニークな化合物コレクションの一部として、基礎研究用途で本製品を提供しています。弊社ではこの製品を分析しておりません。必要に応じて、ご使用前にお客様ご自身で物質の同定と純度を確認することをお勧めします。また、ご購入後の返品や交換は致しかねます。

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable