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P4978

Sigma-Aldrich

Phosphatase, Alkaline from calf intestine

buffered aqueous glycerol solution

Sinonimo/i:

CIAP, CIP, Orthophosphoric-monoester phosphohydrolase (alkaline optimum)

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About This Item

Numero CAS:
9001-78-9
Classificazione EC (Enzyme Commission):
3.1.3.1 (BRENDA, IUBMB)
Numero MDL:
MFCD00131849
Codice UNSPSC:
12352204
eCl@ss:
42010105
NACRES:
NA.53

Grado

for molecular biology

Forma fisica

buffered aqueous glycerol solution

PM

~80 kDa

Concentrazione

≥10,000 units/mL

N° accesso UniProt

P19111

Attività estranea

DNase, RNase, none detected

Condizioni di spedizione

wet ice

Temperatura di conservazione

−20°C

Informazioni sul gene

cow ... ALPI(280993)

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Descrizione generale

Alkaline phosphatase is a phosphomonoesterase that catalyzes the cleavage of terminal phosphates from nucleic acids. The free 5′-OH can be phosphorylated with polynucleotide kinase and γ32P-ATP to produce γ32P-end-labeled nucleic acids. Linearized cloning vectors can be prevented from recircularizing by dephosphorylation with alkaline phosphatase.

Applicazioni

Commonly used to remove the 5′-terminal phosphate from nucleic acids during molecular cloning reactions.

Componenti

Alkaline phosphatase is provided in a solution of 10 mM Tris-HCl (pH 8.2), 50 mM KCl, 1 mM MgCl2, and 0.1 mM ZnCl2, in 50% (w/v) glycerol. 10X CIP Buffer, Product Number C3225, is included.

Definizione di unità

One unit will hydrolyze 1 μmole of p-nitrophenyl phosphate per min at 37 °C.

Altre note

Alkaline phosphatase in 5 mM EDTA (pH 8) will be irreversibly heat inactivated at 75 °C for 10 minutes.

Inibitore

N° Catalogo
Descrizione
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D7291

Deoxyribonuclease I RNase-free solution from bovine pancreas

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P6911

Proteasi, BioReagent, DNase, RNase, and nickase, none detected (No RNase.)

LIG1

DNA Ligation Kit

Substrato

N° Catalogo
Descrizione
Determinazione del prezzo

Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Certificati d'analisi (COA)

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Protocolli

Calf Intestinal (CIP) Alkaline Phosphatase for Nucleic Acid Dephosphorylation

CIP is used to remove 5’-phosphate groups from DNA, RNA and both ribo and deoxy-ribonucleoside triphosphates. Detailed protocol on how to dephosphorylate DNA.

Diethanolamine Assay for the Enzymatic Assay of Alkaline Phosphatase (EC 3.1.3.1)

Enzymatic Assay of Alkaline Phosphatase, Diethanolamine Assay (EC 3. 1. 3. 1)

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