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D9380

Sigma-Aldrich

DNA Polymerase I from Escherichia coli lysogenic for NM 964

buffered aqueous glycerol solution

Sinonimo/i:

Kornberg Polymerase

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About This Item

Numero CAS:
9012-90-2
Classificazione EC (Enzyme Commission):
2.7.7.7 (BRENDA, IUBMB)
Numero MDL:
MFCD00130924
Codice UNSPSC:
12352204
NACRES:
NA.53

Grado

for molecular biology

Livello qualitativo

200

Forma fisica

buffered aqueous glycerol solution

PM

109 kDa

Concentrazione

5,000-15,000 units/mL

N° accesso UniProt

P00582

Attività estranea

Endonuclease, none detected

Condizioni di spedizione

wet ice

Temperatura di conservazione

−20°C

Informazioni sul gene

Escherichia coli K12 ... polA(948356)

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Descrizione generale

DNA polymerase I (holoenzyme) has 5′→3′ and 3′→5′ exonuclease activities in addition to its synthetic activity. This bifunctional activity enables the enzyme to use nicks or gaps in double stranded DNA as starting points for DNA synthesis. The 5′→3′ exonuclease activity degrades the DNA strand complementary to the template strand beginning at the nick. DNA synthesis begins at the 3′-end of the nick and produces a new strand of DNA complementary to the template. The net result is the movement of the polymerase along the template strand (nick translation) until the DNA complementary to the template (from the site of the original nick to the 5′-end of the template strand) is replaced.

Applicazioni

DNA Polymerase I from Escherichia coli has been used to study the effects of the anti-tumor drug cis-diaminedichloroplatinum (II) on the enzyme activity.
Suitable for:
  • Highly specific DNA probes by nick translation
  • In vitro synthesis of complementary cDNA strand
  • In vitro synthesis of DNA
  • Produce blunt ends from 5′ and 3′ overhangs

Componenti

DNase Polymerase I is supplied in a solution of 50% glycerol containing 100 mM potassium phosphate buffer (pH 6.5), and 1 mM dithiothreitol.

Definizione di unità

One unit converts 10 nanomoles of deoxyribonucleoside triphosphates into acid insoluble material in 30 min at 37 °C.

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P6911

Proteasi, BioReagent, DNase, RNase, and nickase, none detected (No RNase.)

LIG1

DNA Ligation Kit

Pittogrammi

Health hazard
GHS08

Avvertenze

Danger

Indicazioni di pericolo

H334

Consigli di prudenza

P261 - P284 - P501

Classi di pericolo

Resp. Sens. 1

Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Lehman, I.R., et al.
The Enzymes, 14A, 16-38 (1981)
Inhibition of Escherichia coli DNA polymerase-I by the anti-cancer drug cis-diaminedichloroplatinum(II): what roles do polymerases play in cis-platin-induced cytotoxicity?
Rebecca K
Febs Letters (1999)
Micrococcus luteus deoxyribonucleic acid polymerase. Studies of the enzymic reaction and properties of the deoxyribonucleic acid product.
S J Harwood et al.
The Journal of biological chemistry, 245(21), 5614-5624 (1970-11-10)
J M D'Alessio et al.
Nucleic acids research, 16(5), 1999-2014 (1988-03-25)
A simple method for generating cDNA libraries has been described (1) in which RNase H-DNA polymerase I-mediated second-strand cDNA synthesis primes from an RNA oligonucleotide derived from the 5' (capped) end of mRNA. The size of this oligonucleotide and the
H Okayama et al.
Molecular and cellular biology, 2(2), 161-170 (1982-02-01)
A widely recognized difficulty of presently used methods for cDNA cloning is obtaining cDNA segments that contain the entire nucleotide sequence of the corresponding mRNA. The cloning procedure described here mitigates this shortcoming. Of the 10(5) plasmid-cDNA recombinants obtained per
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