R0884
T7 RNA Polymerase
recombinant, expressed in E. coli, buffered aqueous solution
Sinonimo/i:
RNA Polymerase T7, RNA Polymerase, T7 from E. coli HMS 174/pAR1219
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About This Item
Numero CAS:
Numero MDL:
Codice UNSPSC:
12352204
Prodotti consigliati
Ricombinante
expressed in E. coli
Grado
Molecular Biology
for molecular biology
Stato
buffered aqueous solution
PM
98.8 kDa
Concentrazione
10,000-50,000 U/mL
N° accesso UniProt
Attività estranea
DNase and RNase, none detected
Temperatura di conservazione
−20°C
Informazioni sul gene
bacteriophage T7 ... T7p07(1261050)
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Descrizione generale
T7 RNA polymerase is highly specific for the bacteriophage T7 promoter and terminator sequences. It is extensively used to prepare RNA transcripts for stuctural and metabolic studies. The RNA transcripts can be converted to probes for sensitive hybridization detection studies. T7 polymerase and dideoxynucleotides can be used to directly sequence DNA.
Componenti
T7 RNA Polymerase is supplied as a solution of 100 mM NaCl, 50 mM Tris-HCl (pH 7.9), 0.1 mM EDTA, 0.1% Triton X-100, 1 mM DTT, and 50% (v/v) glycerol.
Definizione di unità
One unit will catalyze the incorporation of 1 nmol of rNTP into acid-precipitable material in 60 min at 37°C.
Risultati analitici
Activity assay: 40 mM Tris-HCl, pH 7.9, 6 mM MgCl2, 4 mM spermidine, 10 mM DTT, 0.5 μM each rNTP + 10 μCi α-32P-UTP, 3-10 units of enzyme, and 1 μg of a 350 bp template are incubated for 10 min at 37°C in a total volume of 100 μl. Typical results are ≥50% incorporation of labeled nucleotide into ≥90% full-length transcript.
Prodotti correlati
N° Catalogo
Descrizione
Determinazione del prezzo
Codice della classe di stoccaggio
10 - Combustible liquids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
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I clienti hanno visto anche
Characterization of T7-specific ribonucleic acid polymerase. 1. General properties of the enzymatic reaction and the template specificity of the enzyme.
M Chamberlin et al.
The Journal of biological chemistry, 248(6), 2235-2244 (1973-03-25)
Byung-Chun Kim et al.
Journal of microbiology and biotechnology, 23(2), 189-194 (2013-02-16)
In a study of hydrogen-producing bacteria, strain T4384 was isolated from rice field samples in the Republic of Korea. The isolate was identified as Enterobacter sp. T4384 by phylogenetic analysis of 16S rRNA and rpoB gene sequences. Enterobacter sp. T4384
Ran Furman et al.
FEBS letters, 587(6), 614-619 (2013-02-19)
Transcription factor DksA contains a four-Cys Zn(2 +)-finger motif thought to be responsible for structural integrity and the relative disposition of its domains. Pseudomonas aeruginosa encodes an additional DksA paralog (DksA2) that is expressed selectively under Zn(2+) limitation. Although DksA2
Looking for a promoter in 3D.
Vladimir Svetlov et al.
Nature structural & molecular biology, 20(2), 141-142 (2013-02-06)
Yuko Murayama et al.
Acta crystallographica. Section F, Structural biology and crystallization communications, 69(Pt 2), 174-177 (2013-02-07)
DNA-dependent RNA polymerase (RNAP) synthesizes RNA complementary to the template DNA. During transcript elongation, RNAP often undergoes backward translocation ('backtracking') by dissociating the 3' end of the nascent RNA transcript from the template DNA. While the backtracked state of RNAP
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