516620

Peroxynitrite

liquid, biological oxidizing agent, Calbiochem^®

• Sinonimo/i: Nitroxyl anion, Peroxynitrous acid
• Codice UNSPSC: 51111800
• NACRES: NA.77

Proprietà

• Nome del prodotto: Peroxynitrite, Synthesized from isoamyl nitrite and hydrogen peroxide.
• Livello qualitativo: 100
• Stato: liquid
• Produttore/marchio commerciale: Calbiochem^®
• Condizioni di stoccaggio: OK to freeze; avoid repeated freeze/thaw cycles; protect from light
• Colore: yellow
• Condizioni di spedizione: wet ice
• Temperatura di conservazione: −70°C

Descrizione

Descrizione generale

Note: 1 set = 2 x 1 ml.

Synthesized from isoamyl nitrite and hydrogen peroxide. Peroxynitrite (ONOO–) is a strong biological oxidizing agent that reacts with DNA, membrane phospholipids, sulfhydryl groups, and tyrosine. It has been implicated in a variety of patho-physiological conditions including acute respiratory distress syndrome (ARDS), arthritis, atherosclerosis, inflammatory bowel disease, ischemia-reperfusion, N-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-mediated neurotoxicity, and septic shock. This product decomposes or isomerizes at pH 8 or less; the half-life at pH 7.5 is ~1.5 s. ε^{302 nm} = 1670^M-1cm^-1 in 0.1 MNaOH.

Synthesized from isoamyl nitrite and hydrogen peroxide. Peroxynitrite (ONOO^–) is a cell-permeable strong biological oxidizing agent that reacts with DNA, membrane phospholipids, sulfhydryl groups, and tyrosine. Has been implicated in a variety of pathophysiological conditions including acute respiratory distress syndrome (ARDS), arthritis, atherosclerosis, inflammatory bowel disease, ischemia-reperfusion, N-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-mediated neurotoxicity, and septic shock.

Azioni biochim/fisiol

Cell permeable: yes

Primary Target
Oxidizing agent that reacts with DNA, membrane phospholipids, sulfhydryl groups, and tyrosine

Product does not compete with ATP.

Reversible: no

Confezionamento

Packaged under inert gas

Attenzione

Toxicity: Oxidizer (K)

Stato fisico

Supplied in 4.7% NaOH at 160-200 mM.

Nota sulla preparazione

Peroxynitrite is soluble in aqueous buffers that have been pre-purged with an inert gas and pre-cooled to 4°C.

Ricostituzione

Protect from heat, light, metal ions, and air. Peroxynitrite is unstable; thaw rapidly, dispense into aliquots, purge tubes with an inert gas, and freeze -70°C. Activity decreases ~2% /day at -20°C.

Altre note

Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.

Marla, S.S., et al. 1997. Proc. Natl. Acad. Sci. USA94, 14243.
Sakuma, S., et al. 1997. Biochem. Biophys. Res. Commun. 230, 476.
Lymar, S.V., et al. 1996. Biochemistry 35, 7855.
Uppu, R.M., and Pryor, W.A. 1996. Methods Enzymol. 269, 322.
Ischiropoulos, H., and al-Medhi, A.B. 1995. FEBS Lett. 364, 279.

Note legali

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informazioni sulla sicurezza

• Pittogrammi: GHS08,GHS05
• Avvertenze: Danger
• Indicazioni di pericolo: H290,H314,H341,H351
• Consigli di prudenza: P202 - P234 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Classi di pericolo: Carc. 2 - Eye Dam. 1 - Met. Corr. 1 - Muta. 2 - Skin Corr. 1B
• Codice della classe di stoccaggio: 5.1B - Oxidizing hazardous materials
• Classe di pericolosità dell'acqua (WGK): WGK 2
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable