D5921
Dulbecco′s Modified Eagle′s Medium - low glucose
With 1000 mg/L glucose, and sodium bicarbonate, without L-glutamine and phenol red, liquid, sterile-filtered, suitable for cell culture
Sinonimo/i:
DME, DMEM
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About This Item
Livello qualitativo
Sterilità
sterile-filtered
Forma fisica
liquid
tecniche
cell culture | mammalian: suitable
Impurezze
endotoxin, tested
Componenti
phenol red: no
sodium pyruvate: no
glucose: low
HEPES: no
L-glutamine: no
NaHCO3: yes
Condizioni di spedizione
ambient
Temperatura di conservazione
2-8°C
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Categorie correlate
Descrizione generale
Dulbecco′s Modified Eagle′s medium (DME) is a modification of Basal Medium Eagle (BME), which has a four-fold higher concentration of amino acids and vitamins along with additional supplementary components. The first original DME formula was reported to culture embryonic mouse cells comprising 1,000 mg/L of glucose. An alteration with 4,500 mg/L glucose is optimal in cultivating certain cell types. DME has been shown to improve the stability of the medium.
Applicazioni
Dulbecco′s Modified Eagle′s medium-low glucose has been used to culture:
- living colon cancer SW620 cells
- human breast carcinoma cell lines MCF-7(13), KPL and MDA-MB-231
- mammal and canine derived adipose-derived mesenchymal stem cells
Ricostituzione
Supplement with 0.584 g/L L-glutamine.
Altre note
Investigators who work with stem cells or cell clones frequently prefer media without phenol red.
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Avvertenze
Warning
Indicazioni di pericolo
Consigli di prudenza
Classi di pericolo
Skin Sens. 1
Codice della classe di stoccaggio
12 - Non Combustible Liquids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
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